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2 protocols using tall 104

1

Cell Line Cultivation and Authentication

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HCA-7, HT-29, 293T, and TALL-104 cell lines were obtained from ATCC (Manassas, VA). HCA-7 and HT-29 cells were cultured in McCoy’s 5A medium with 10% FBS. 293T cells were cultured in DMEM medium with 10% FBS. TALL-104 cells were cultured in RPMI1640 medium supplemented with recombinant IL-2 (Peprotech, Cat#200-02) and 20% FBS (Gibco, Cat#10082147). HCEC-1CT cells were cultured in DMEM medium (Corning, Cat#10-013-cv) supplemented with 25 ng/mL EGF (Invitrogen, Cat#PHG0311), 1 μg/mL hydrocortisone (Sigma, Cat#H0888), 1× insulin -transferrin-sodium selenite (Gibco, Cat# 41400045), 50 μg/mL gentamicin (Gibco, Cat#15750-060), and 2% cosmic calf serum (HyClon, Cat# SH30087.02). All cell lines have been tested by MycoProbe Mycoplasma Detection Kit (R&D) and also authenticated before the experiment according ATCC STR database.
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2

Cell Line Cultivation and Authentication

Check if the same lab product or an alternative is used in the 5 most similar protocols
HCA-7, HT-29, 293T, and TALL-104 cell lines were obtained from ATCC (Manassas, VA). HCA-7 and HT-29 cells were cultured in McCoy’s 5A medium with 10% FBS. 293T cells were cultured in DMEM medium with 10% FBS. TALL-104 cells were cultured in RPMI1640 medium supplemented with recombinant IL-2 (Peprotech, Cat#200-02) and 20% FBS (Gibco, Cat#10082147). HCEC-1CT cells were cultured in DMEM medium (Corning, Cat#10-013-cv) supplemented with 25 ng/mL EGF (Invitrogen, Cat#PHG0311), 1 μg/mL hydrocortisone (Sigma, Cat#H0888), 1× insulin -transferrin-sodium selenite (Gibco, Cat# 41400045), 50 μg/mL gentamicin (Gibco, Cat#15750-060), and 2% cosmic calf serum (HyClon, Cat# SH30087.02). All cell lines have been tested by MycoProbe Mycoplasma Detection Kit (R&D) and also authenticated before the experiment according ATCC STR database.
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