Total RNA was isolated using RNeasy kits and reverse transcribed into cDNA with Omniscript RT kit (both Qiagen). QPCR was performed using the Mastercycler® ep realplex2 (link) System (Eppendorf). Platimum® Quantitative PCR SuperMix-UDG (Invitrogen) and TaqMan probes and primers were used for Actb, Il9, Il10, Il13, Batf (IDT) (Supplementary Table 7 ). RT2 SYBR® Green qPCR Mastermix (Qiagen) and primer sets were used for Actb, Batf3 (IDT). mRNA expression of target genes was normalized to the expression of Actb. The relative gene expression was calculated by the 2-ΔΔCt method.
Mastercycler ep realplex2 link system
Manufactured by Eppendorf
The Mastercycler® ep realplex2 System is a real-time PCR thermal cycler designed for quantitative gene expression analysis. It provides precise temperature control and optical detection for reliable and reproducible results.
Automatically generated - may contain errors
2 protocols using mastercycler ep realplex2 link system
1
Quantitative PCR Expression Analysis
2
Quantitative PCR Expression Analysis
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Total RNA was isolated using RNeasy kits and reverse transcribed into cDNA with Omniscript RT kit (both Qiagen). QPCR was performed using the Mastercycler® ep realplex2 (link) System (Eppendorf). Platimum® Quantitative PCR SuperMix-UDG (Invitrogen) and TaqMan probes and primers were used for Actb, Il9, Il10, Il13, Batf (IDT) (Supplementary Table 7 ). RT2 SYBR® Green qPCR Mastermix (Qiagen) and primer sets were used for Actb, Batf3 (IDT). mRNA expression of target genes was normalized to the expression of Actb. The relative gene expression was calculated by the 2-ΔΔCt method.
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