Levels of IL-6, IL-10, IL-12, and tumor necrosis factor-α (TNF-α) were determined using the Luminex/Bioplex assay 200 system (Bio-Rad) per the manufacturer's protocol. The limit of detection for each cytokine was <5 pg/mL. The cytokine levels were normalized to protein present in a cell-free preparation of each sample measured by Bradford Assay (Bio-Rad).
Luminex bioplex assay 200 system
Manufactured by Bio-Rad
The Luminex/Bioplex assay 200 system is a multiplex analysis platform that enables the simultaneous detection and quantification of multiple analytes from a single sample. The system utilizes fluorescent-labeled beads coated with specific capture antibodies to bind and identify target molecules in the sample. The system is capable of performing a wide range of assays, including protein, nucleic acid, and cell-based analyses.
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Lab products found in correlation
2 protocols using luminex bioplex assay 200 system
1
Quantification of Inflammatory Cytokines
2
Cytokine Profiling in Thrombosis
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Levels of IL-1β, IL-6, IL-10, and IL-12 present in thrombus from the stasis and nonstasis VT murine models were determined using the Luminex/Bio-Plex assay 200 system (Bio-Rad, Carlsbad, Calif) per the manufacturer's protocol. The limit of detection for each cytokine was 0.5 ρg/mL. The cytokine levels were normalized to protein present in a cell-free preparation of each sample measured by Bradford assay (Bio-Rad).
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