DFCs, iDFCs and dDFCs were digested and then resuspended in PBS containing 10%FBS to a final concentration of 1*107 cells/ml. Distribute 100 μl aliquots of the cell suspension into each tube. Add antibodies to cells and incubate for 20 min in the dark. Antibodies used were FITC-conjugated mouse anti- human CD34 (BD Biosciences, 555821; 20 μl), phycoerythrin(PE)-conjugated mouse anti-human CD73 (BD Biosciences, 550257; 20 μl), FITC-conjugated mouse anti-human CD105 (BD Biosciences, 561443; 5 μl) and FITC-conjugated anti-human Strol-1. Finally, flow cytometry(BD Biosciences) was used to analyze the stained cells.
Fitc conjugated mouse anti human cd105
Manufactured by BD
FITC-conjugated mouse anti-human CD105 is a monoclonal antibody that binds to the CD105 (endoglin) antigen expressed on the surface of human cells. The antibody is labeled with the fluorescent dye fluorescein isothiocyanate (FITC), which allows for detection and analysis of CD105-positive cells using flow cytometry or other fluorescence-based methods.
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2 protocols using fitc conjugated mouse anti human cd105
1
Characterization of Mesenchymal Stem Cells
2
Flow Cytometric Characterization of HDFCs
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For flow cytometry analysis, HDFCs were harvested at passage 2, 106 suspended cells were washed and stained by incubation with the following antibodies and corresponding isotype controls: FITC-conjugated mouse antihuman CD34 (BD Biosciences, San Diego, CA), FITC-conjugated mouse antihuman CD45 (BD Biosciences), FITC-conjugated mouse antihuman CD90 (BD Biosciences) and FITC-conjugated mouse antihuman CD105 (BD Biosciences), and after 30 min of incubation and washing twice with PBS subsequently centrifuged for 5 min at 250g, and cells were resuspended in 500 mL PBS and filtered through a 100-μm strainer. Samples and controls were measured by a BD Flow Cytometer (BD Biosciences), and data analysed using FlowJo 7.6.1 software.
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