Sod1 antibody

Manufactured by Abcam

Sourced in United Kingdom

The SOD1 antibody is a laboratory reagent used for the detection and analysis of superoxide dismutase 1 (SOD1) protein in various biological samples. SOD1 is an important antioxidant enzyme that plays a crucial role in protecting cells from oxidative stress. This antibody can be utilized in techniques such as Western blotting, immunohistochemistry, and immunoprecipitation to study the expression and localization of SOD1 in various experimental systems.

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Lab products found in correlation

P67phox, by Santa Cruz Biotechnology (1 mentions) Trizol rna extraction reagent, by Thermo Fisher Scientific (1 mentions) Cebpd, by Santa Cruz Biotechnology (1 mentions) Gfap antibody, by Thermo Fisher Scientific (1 mentions) Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions) Superscript 3, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Cytiva (1 mentions) Hemoglobin, by Merck Group (1 mentions) Alexa fluor 488 goat anti rabbit secondary antibody, by Thermo Fisher Scientific (1 mentions) C fos, by Cell Signaling Technology (1 mentions) Dmem f12, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions) 2 7 dichlorofluorescein diacetate dcf da, by Merck Group (1 mentions) β actin, by Santa Cruz Biotechnology (1 mentions) Ho 1 antibody, by Stressgen (1 mentions)

2 protocols using sod1 antibody

Oxidative Stress Signaling Pathway

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The CEBPD, p67^phox, and nitrotyrosine antibodies and TETA were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The GFAP antibody was purchased from Invitrogen (Carlsbad, CA, USA). The p47^phox antibody was purchased from MDBio Inc. (Taipei, Taiwan). The SOD1 antibody was purchased from Abcam plc. (Cambridge, MA). The Dulbecco's modified Eagle's medium (DMEM), TRIzol RNA extraction reagent, and SuperScript™ III were purchased from Invitrogen (Carlsbad, CA, USA). Fetal bovine serum (FBS) was purchased from HyClone Laboratories (Logan, UT, USA). All oligonucleotides were synthesized by MDBio Inc. (Taipei, Taiwan).

Wang S.M., Lim S.W., Wang Y.H., Lin H.Y., Lai M.D., Ko C.Y, & Wang J.M. (2018). Astrocytic CCAAT/Enhancer-binding protein delta contributes to reactive oxygen species formation in neuroinflammation. Redox Biology, 16, 104-112.

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Examining Cellular Antioxidant Mechanisms

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Compound (−)-epicatechin, hemoglobin, MTT, and 2′,7′-dichlorofluorescein diacetate (DCF-DA) were purchased from Sigma Aldrich (Allentown, PA, USA). D-Hanks and 0.25% trypsin were purchased from Quality Biological (Gaithersburg, MD, USA). DMEM/F12, FBS, and Alexa Fluor 488 goat anti-rabbit secondary antibody were obtained from Life Technologies (Carlsbad, CA, USA). Antibodies of Nrf2 (H-300), Lamin a/c, and β-actin were obtained from Santa Cruz Biotechnology (Dallas, TX, USA). SOD1 antibody was purchased from Abcam (Cambridge, UK). HO1 antibody was purchased from Stressgen, (San Diego, CA, USA). Antibodies of phospho-SAPK/JNK, SAPK/JNK, c-jun, c-fos, and peroxidase-coupled goat anti-rabbit or anti-mouse secondary antibody were purchased from Cell Signaling Technology (Danvers, MA, USA).

Lan X., Han X., Li Q, & Wang J. (2016). (−)-Epicatechin, a Natural Flavonoid Compound, Protects Astrocytes Against Hemoglobin Toxicity via Nrf2 and AP-1 Signaling Pathways. Molecular neurobiology, 54(10), 7898-7907.

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