Log In Sign up
The largest database of trusted experimental protocols

Diur 500

Manufactured by BioAssay Systems
Visit Supplier
Sourced in United States

DIUR-500 is a compact and versatile spectrophotometer designed for accurate measurements of optical density. It features a wide wavelength range, high-precision optics, and user-friendly controls, making it suitable for a variety of laboratory applications.

Automatically generated - may contain errors

Lab products found in correlation

Dict 500, by BioAssay Systems (5 mentions) Albuwell m, by Exocell (2 mentions) E80 129, by Fortis Life Sciences (1 mentions) Ealt 100, by BioAssay Systems (1 mentions) Eastr 100, by BioAssay Systems (1 mentions)

Spelling variants of this product

QuantiChrom™ Urea Assay Kit (DIUR-500) (9 citations) DIUR 500 kit (3 citations) Quantichrom DIUR 500 kit (2 citations)

6 protocols using diur 500

1

Serum Creatinine and BUN Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse blood samples collected from heart puncture at sacrifice or from the tail vein were clotted and centrifuged to collect serum. Serum creatinine concentration was measured by the picric acid method with a commercial kit (DICT-500, BioAssay Systems), following the manufacturer’s instructions. BUN was measured with a commercial kit (DIUR-500, BioAssay Systems) following the manufacturer’s instructions.
Cao X., Wang J., Zhang T., Liu Z., Liu L., Chen Y., Li Z., Zhao Y., Yu Q., Liu T., Nie J., Niu Y., Chen Y., Yang L, & Zhang L. (2022). Chromatin accessibility dynamics dictate renal tubular epithelial cell response to injury. Nature Communications, 13, 7322.
+ Open protocol
+ Expand
2

Kidney Function Biomarker Assays

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Plasma urea level was detected with a urea assay kit (DIUR-500; BioAssay Systems, Hayward, CA, USA). Plasma and urinary creatinine levels were detected using a creatinine assay kit (DICT-500; BioAssay Systems). Urinary albumin concentration was measured with a mouse-specific microalbuminuria ELISA kit (Albuwell M; Exocell, Philadelphia, PA, USA). All kidney function parameters were measured according to manufacturers' instructions 16 (link).
Lai L., Li Y., Liu J., Luo L., Tang J., Xue J, & Liu T. (2021). Bovine serum albumin aggravates macrophage M1 activation and kidney injury in heterozygous Klotho-deficient mice via the gut microbiota-immune axis. International Journal of Biological Sciences, 17(3), 742-755.
+ Open protocol
+ Expand
3

Renal Function Parameters Measurement

Check if the same lab product or an alternative is used in the 5 most similar protocols
Plasma urea level was detected with a urea assay kit (DIUR-500; BioAssay Systems, Hayward, CA, USA). Plasma and urinary creatinine levels were detected with a creatinine assay kit (DICT-500; BioAssay Systems). Urinary albumin concentration was measured with a mouse-specific microalbuminuria ELISA kit (Albuwell M; Exocell, Philadelphia, PA, USA). All renal function parameters were measured according to manufacturers’ instructions.
Gao D., Wang S., Lin Y, & Sun Z. (2020). In vivo AAV delivery of glutathione reductase gene attenuates anti-aging gene klotho deficiency-induced kidney damage. Redox Biology, 37, 101692.
+ Open protocol
+ Expand
4

Urea and Albumin Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
After running the experiments, all media were collected and stored at −80°C. The urea concentration was measured using the urea assay kit (DIUR-500; Bioassay Systems LLC, Hayward, CA, USA). The albumin concentration was assayed with the human albumin ELISA quantitation set (E80-129; Bethyl Laboratories, Montgomery, TX, USA). All results were analyzed with CurveExpert 1.3 software and fitted with logistic model with r2>0.99.
Zhang X., Lu J., He B., Tang L., Liu X., Zhu D., Cao H., Wang Y, & Li L. (2016). A tryptophan derivative, ITE, enhances liver cell metabolic functions in vitro. International Journal of Molecular Medicine, 39(1), 101-112.
+ Open protocol
+ Expand
5

Evaluating Mouse Liver and Kidney Function

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse liver functions were assessed by measuring serum alanine transaminase and aspartate transaminase (EALT-100 and EASTR-100, respectively, EnzyChrom, BioAssay Systems) according to the manufacturer’s protocol. Mouse kidney functions were assessed by measuring serum creatinine and blood urea nitrogen levels (DICT-500 and DIUR-500, respectively, Quantichrome, BioAssay Systems) according to the manufacturer’s protocol. Samples with insufficient volumes were diluted with assay buffer provided from the manufacturer. All assays were run with biological replicates in technical triplicates normalized to control levels.
Ishak Gabra M.B., Yang Y., Li H., Senapati P., Hanse E.A., Lowman X.H., Tran T.Q., Zhang L., Doan L.T., Xu X., Schones D.E., Fruman D.A, & Kong M. (2020). Dietary glutamine supplementation suppresses epigenetically-activated oncogenic pathways to inhibit melanoma tumour growth. Nature Communications, 11, 3326.
+ Open protocol
+ Expand
6

Comprehensive Kidney Injury Assessment

Check if the same lab product or an alternative is used in the 5 most similar protocols
Creatinine was tested (ml037726, Mlbio, Shanghai, China) using tissues of both insulted left kidney and the contralateral right kidney. In addition, mice serum was used to detected the systemic serum creatinine (SCr) (DICT-500, BioAssay System, Hayward, CA, USA), urea (DIUR-500, BioAssay System, Hayward, CA, USA), alanine aminotransferease (ALT) (ml063179, Mlbio, Shanghai, China), and aspartate aminotransferase (AST) (ml058577, Mlbio, Shanghai, China).
Jiang Y., Cai C., Zhang P., Luo Y., Guo J., Li J., Rong R., Zhang Y, & Zhu T. (2022). Transcriptional profile changes after treatment of ischemia reperfusion injury-induced kidney fibrosis with 18β-glycyrrhetinic acid. Renal Failure, 44(1), 660-671.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!