The sample pad, conjugate pad, nitrocellulose membrane (NC), absorbent pad and backing card were purchased from the Jie Yi Biotechnology Co., Ltd. (Shanghai, China). The streptavidin-immobilized gold nanoparticles (SA-G), rabbit anti-fluorescein antibody (anti-FITC) and biotinylated bovine serum albumin (biotin-BSA) were purchased from the Resenbio Co., Ltd. (XiAn, China). The QIAamp DNA Stool Mini Kit and QIAamp DNA Mini Kit (QIAamp DNA minikits; Qiagen, Hilden, Germany) were purchased from Qiagen (Beijing, China). LoopampTM Fluorescent Detection Reagent (FD) and the Loopamp kits were purchased from Eiken Chemical (Beijing, China).
Loopamp fluorescent detection reagent fd
Manufactured by Eiken Chemical
Sourced in China
The LoopampTM Fluorescent Detection Reagent (FD) is a laboratory product developed by Eiken Chemical. It is designed to detect and quantify the presence of specific target sequences during nucleic acid amplification reactions, such as loop-mediated isothermal amplification (LAMP). The reagent emits a fluorescent signal when bound to the amplified target, allowing for real-time monitoring of the amplification process.
Automatically generated - may contain errors
4 protocols using loopamp fluorescent detection reagent fd
1
Rapid Fluorescence-based Diagnostic Assay
2
Loop-Mediated Isothermal DNA Amplification
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LoopampTM Fluorescent Detection Reagent (FD) and the Loopamp kits were purchase from Eiken Chemical (Tokyo, Japan, and Beijing, China). The DNA extraction kits (QIAamp DNA Mini Kits) were purchased from Qiagen (Beijing, China), and the Nb.BsrDI was purchased from New England Biolabs (Beijing, China).
3
Rapid Immunoassay for Biomolecule Detection
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The sheep anti-digoxigenin antibody (anti-Dig), the streptavidin-immobilized 30 nm gold nanoparticles (SA-G), and biotinylated bovine serum albumin (biotin-BSA) were purchased from the Resenbio Co., Ltd. (Xi’an, People’s Republic of China). The backing card, absorbent pad, nitrocellulose (NC) membrane, conjugate pad, and sample pad were purchased from the Jie Yi Biotechnology Co., Ltd. (Shanghai, People’s Republic of China). The Loopamp kits and Loopamp™ Fluorescent Detection Reagent (FD) were purchased from Eiken Chemical (Beijing, People’s Republic of China). The DNA extraction kits (QIAamp DNA minikits; Qiagen, Hilden, Germany) were purchased from Qiagen (Beijing, People’s Republic of China).
4
Oligomer Synthesis and Purification
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All of the oligomers were commercially synthesized and purified by Tsingke (Beijing, China). The Loopamp DNA amplification kits and Loopamp™ Fluorescent Detection Reagent (FD) were purchased from Eiken Chemical Co. Ltd. (Beijing, China). The DNA Extraction Kits (QIAamp DNA minikits) and QIAquick Gel Extraction Kit (QIAGEN, Hidlen, Germany) were purchased from Qiagen (Beijing, China).
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