7200 gcqtof ms

Manufactured by Agilent Technologies

Sourced in United States

The 7200 GCqTOF MS is a gas chromatography-quadrupole time-of-flight mass spectrometer. It is a high-resolution analytical instrument designed for the identification and quantification of chemical compounds.

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Lab products found in correlation

7890a series, by Agilent Technologies (1 mentions) J w scientific hp5 ms, by Agilent Technologies (1 mentions)

2 protocols using 7200 gcqtof ms

Quantitative Determination of α-Ketoglutarate in Serum by GC/MS

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Aplha-ketoglutarate was quantitatively determined in serum samples by GC/MS at the Centre for Omic Sciences (Rovira i Virgili University). The methodology used has been reported elsewhere [10 (link)]. Samples were analysed in a 7890A Series gas chromatograph coupled to a 7200 GCqTOF MS (Agilent Technologies, Santa Clara, U.S.A.). The chromatographic column was a J&W Scientific HP5-MS (30 m x 0.25 mm i.d., 0.25 μm film) (Agilent Technologies). The calibration curve showed good linearity in the studied range of 0.13 to 10 mg/L, with a determination coefficient (R²) of 0.9993. The extraction recovery was 99.7%, while accuracy was 101.7%. The intraday and interday precision had a relative standard deviation of, respectively, 4.1% and 4.8% (RSD, n = 3). MDL was 0.05 mg/L, while MQL was 0.13 mg/L.

Aragonès G., Auguet T., Berlanga A., Guiu-Jurado E., Martinez S., Armengol S., Sabench F., Ras R., Hernandez M., Aguilar C., Colom J., Sirvent J.J., Del Castillo D, & Richart C. (2016). Increased Circulating Levels of Alpha-Ketoglutarate in Morbidly Obese Women with Non-Alcoholic Fatty Liver Disease. PLoS ONE, 11(4), e0154601.

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Quantitative analysis of primary metabolites

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Relative levels of primary metabolites were measured as described in Hasler-Sheetal et al. (2016) (link). In brief, 50-mg FW samples (2nd leaves from 4-month-old plants) from six independent T₀ CBM20-expressing plants and six vector control plants were extracted for 2 min in an ultrasound bath followed by 15 min on a thermo-shaker (both at 4°C) in 1 ml methanol/acetonitrile/water (4:4:2 v/v/v) chilled to –20°C and spiked with 0.4 mg l^{–1 13}C6 sorbitiol and reserpine as internal standards. After centrifugation at 16 000 g for 5 min, the relative metabolite levels were analysed by GC-quadrupole time-of-flight MS (7200 GC-QTOF MS, Agilent Technologies).

Zhong Y., Sagnelli D., Topbjerg H.B., Hasler-Sheetal H., Andrzejczak O.A., Hooshmand K., Gislum R., Jiang D., Møller I.M., Blennow A, & Hebelstrup K.H. (2019). Expression of starch-binding factor CBM20 in barley plastids controls the number of starch granules and the level of CO2 fixation. Journal of Experimental Botany, 71(1), 234-246.

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