100–200 µl of homogenized spleen or peripheral blood was treated with 2 ml of EL buffer (QIAGEN) for 1 min to lyse red blood cells. The reaction was quenched by adding 5–6 volume of chilled PBS. After centrifugation and removing supernatant, cells were stained with specific surface marker antibodies. The following antibodies (BD Biosciences) were used: V450 mouse anti‐human CD19 (BD 560353), PE rat‐anti‐mouse CD45 (BD 553081), PE‐Cy7 mouse anti‐human CD34 (BD 348791), and FITC mouse anti‐human CD45 (BD 555482). The cells were sorted using FACS Aria Fusion instrument. FACS data were analyzed using FlowJo software.
Pe cy7 mouse anti human cd34
Manufactured by BD
Sourced in United States
The PE‐Cy7 mouse anti‐human CD34 is a fluorochrome-conjugated monoclonal antibody that binds to the CD34 antigen, which is expressed on the surface of hematopoietic stem and progenitor cells. This product can be used in flow cytometry applications to identify and analyze CD34-positive cell populations.
Automatically generated - may contain errors
2 protocols using pe cy7 mouse anti human cd34
1
Isolation and Characterization of Immune Cells
2
Immunophenotyping of CD34+ Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells suspended in Recommended Medium were labeled with antibodies at 4°C for 30 min. Antibodies used were PE-Cy™7 Mouse Anti-Human CD34 (BD), PE anti-human CD43 (BioLegend, USA), and PE anti-human CD31 (BioLegend). After staining, cells were analyzed by Cytomics™FC 500 (Beckman, USA) with FlowJo software (Tree Star, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!