Log In Sign up
The largest database of trusted experimental protocols

Scanr inverted microscope

Manufactured by Olympus
Visit Supplier

The ScanR inverted microscope is a laboratory instrument designed for imaging and analysis of biological samples. It features an inverted optical configuration, allowing for convenient observation and manipulation of cells and other specimens. The ScanR microscope provides high-quality imaging and is suitable for a variety of applications in life science research and drug discovery.

Automatically generated - may contain errors

Lab products found in correlation

Chemotaxis tool, by Ibidi (2 mentions) Scanr analysis software, by Olympus (1 mentions) Versane, by Thermo Fisher Scientific (1 mentions) Plasma cleaner, by Harrick (1 mentions) Avidin biotin blocking kit, by Vector Laboratories (1 mentions) Axiocam mrm, by Zeiss (1 mentions) Plan neofluar 10x 0.30 objective, by Zeiss (1 mentions) Axiovision software, by Zeiss (1 mentions) Axioplan 2 imaging microscope, by Zeiss (1 mentions)

Spelling variants of this product

Inverted microscope (2417 citations) ScanR (41 citations) ScanR microscope (20 citations) ScanR automated inverted fluorescent microscope system (2 citations) ScanR inverted microscope High-content Screening Station (2 citations)

12 protocols using scanr inverted microscope

1

Wound Healing Assay with Kymograph Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Wound healing assay was performed by Ibidi Culture Inserts (Ibidi) to avoid debrides affecting the quality of the kymograph analysis. Inserts were placed in a 12-well plates and MCF-10A cells were plated in each chamber, 5*104 cells/chamber. 16 hours before starting the experiment, growing media was replaced with fresh complete media containing 2.5 μg/ml of doxycycline to induce RAB5A expression. A cell-free wound area was created by removal of the insert. Cell migration was monitored by Olympus Scan^R inverted microscope with 20X objective (with an additional 1.6x magnification lens).
Images from 10 positions/condition were recorded every 30 seconds over 1 hour period. To measure the dynamic of protrusive structures the Kymograph plugin of ImageJ software was used. Dynamic parameters measured from kymograph images were persistence time (Δt), protrusion rate (Δs1/Δt1) and retraction rate (Δs2/Δt2). The same time interval and ROI length were set in the analysis of each condition.
Malinverno C., Corallino S., Giavazzi F., Bergert M., Li Q., Leoni M., Disanza A., Frittoli E., Oldani A., Martini E., Lendenmann T., Deflorian G., Beznoussenko G.V., Poulikakos D., Haur O.K., Uroz M., Trepat X., Parazzoli D., Maiuri P., Yu W., Ferrari A., Cerbino R, & Scita G. (2017). Endocytic reawakening of motility in jammed epithelia. Nature materials, 16(5), 587-596.
+ Open protocol
+ Expand
2

Automated High-Throughput Microscopy Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols
Images in Fig. 4a,b were acquired with a ScanR inverted microscope (Olympus) using a × 20, 0.75 NA (UPLSAPO × 20) dry objective in an automated fashion. Images were processed and analysed using the propriety ScanR analysis software (Olympus, 2.6.1).
S. Pedersen R., Karemore G., Gudjonsson T., Rask M.B., Neumann B., Hériché J.K., Pepperkok R., Ellenberg J., Gerlich D.W., Lukas J, & Lukas C. (2016). Profiling DNA damage response following mitotic perturbations. Nature Communications, 7, 13887.
+ Open protocol
+ Expand
3

Wound Healing Assay Monitoring

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were seeded in a six-well plate in complete medium and cultured until a uniform monolayer had formed. After overnight starvation, the cell monolayer was scratched with a pipette tip and carefully washed with 1× PBS to remove floating cells and create a cell-free wound area. The closure of the wound was monitored by time-lapse microscopy. An Olympus ScanR inverted microscope with a 10× objective was used to take pictures every 5-10 min over a 24 h period (as indicated in the figure legends). The assay was performed in complete culture medium using an environmental microscope incubator set to 37°C and 5% CO2 perfusion.
Savorani C., Malinverno M., Seccia R., Maderna C., Giannotta M., Terreran L., Mastrapasqua E., Campaner S., Dejana E, & Giampietro C. (2021). A dual role of YAP in driving TGFβ-mediated endothelial-to-mesenchymal transition. Journal of Cell Science, 134(15), jcs251371.
+ Open protocol
+ Expand
4

Single-Cell Migration Assay with HGF

Check if the same lab product or an alternative is used in the 5 most similar protocols
Single-cell migration was monitored as follows. Briefly, siCTR and siNUMB HeLa cells, upon 24 h of interference, were seeded sparsely in a 6-well plate (2 × 104 cells/well) in complete medium. After 24 h, cells were serum starved for 2 h and stimulated with 100 ng/ml HGF. Random cell motility was monitored over a 19-h period. Pictures were taken every 5 min from 10 positions/condition using a motorized Olympus ScanR inverted microscope with 40× objective. All experiments were performed using an environmental microscope incubator set to 37°C and 5% CO2 perfusion. Single cells were manually tracked using the Manual Tracking Tool ImageJ software plugin. Elongation index was calculated as the ratio between the major and the minor axis. Distance and velocity were obtained by Chemotaxis and the Migration Tool ImageJ software plugin.
Zobel M., Disanza A., Senic-Matuglia F., Franco M., Colaluca I.N., Confalonieri S., Bisi S., Barbieri E., Caldieri G., Sigismund S., Pece S., Chavrier P., Di Fiore P.P, & Scita G. (2018). A NUMB–EFA6B–ARF6 recycling route controls apically restricted cell protrusions and mesenchymal motility. The Journal of Cell Biology, 217(9), 3161-3182.
+ Open protocol
+ Expand
5

Visualizing RAB35-Mediated Cell Protrusions

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were seeded in six-wells plate (2 × 104 cells/well) and cultivated in complete medium for 48 h. Where indicated, doxycycline 2.5 mg/ml was added 16 h before the experiment in order to induce RAB35 expression. Medium was refreshed before starting the time-lapse microscopy session. An Olympus ScanR inverted microscope with ×20 objective was used to take phase contrast and fluorescence images every 30″ over a 1 h period, otherwise differently indicated. The assay was performed using an environmental microscope incubator set to 37 °C and 5% CO2 perfusion. Doxycycline was maintained in the media for the total duration of the time-lapse experiment. The number of CDRs per cell, formed during the live imaging session, was counted and reported in dot plot graphs. Each assay was done three times and at least 25 cells/condition were counted in each experiment. Where indicated, LY294002 (20 μM) was added 2 h before imaging. To capture the dynamics of protrusion formation in PDGF-treated or RAB35-ectopically expressing cells, movies generated by time-lapse microscopy were analysed by the Multiple Kymograph plugin of ImageJ software.
Corallino S., Malinverno C., Neumann B., Tischer C., Palamidessi A., Frittoli E., Panagiotakopoulou M., Disanza A., Malet-Engra G., Nastaly P., Galli C., Luise C., Bertalot G., Pece S., Di Fiore P.P., Gauthier N., Ferrari A., Maiuri P, & Scita G. (2018). A RAB35-p85/PI3K axis controls oscillatory apical protrusions required for efficient chemotactic migration. Nature Communications, 9, 1475.
+ Open protocol
+ Expand
6

Chemotactic Response to PDGF-BB Gradient

Check if the same lab product or an alternative is used in the 5 most similar protocols
The ability of cells in responding to a chemotactic stimulus was tested by time-lapse phase contrast microscopy using fibronectin-coated 2D chemotaxis μ-slides from Ibidi. 2 × 10 3 cells were loaded into the central transversal channel and incubated at 37 °C for 30 min in order to allow the adhesion to the substrate. A PDGF-BB gradient (0–20 ng/ml) was generated according to the manufacturer’s instructions. Cell motion was recorded using a motorized Olympus Scan^R inverted microscope with ×10 objective for a 24 h period, taking pictures every 5 min. All the assays were performed using an environmental microscope incubator set to 37 °C and 5% CO2 perfusion. Chemotactic tracks were obtained using the Manual tracking ImageJ software plugin and the chemotaxis plots and migratory parameters were obtained with the Chemotaxis Tool from Ibidi.
Corallino S., Malinverno C., Neumann B., Tischer C., Palamidessi A., Frittoli E., Panagiotakopoulou M., Disanza A., Malet-Engra G., Nastaly P., Galli C., Luise C., Bertalot G., Pece S., Di Fiore P.P., Gauthier N., Ferrari A., Maiuri P, & Scita G. (2018). A RAB35-p85/PI3K axis controls oscillatory apical protrusions required for efficient chemotactic migration. Nature Communications, 9, 1475.
+ Open protocol
+ Expand
7

Micropattern-Guided Cell Locomotion

Check if the same lab product or an alternative is used in the 5 most similar protocols
Micropatterns of fibronectin-coated lines (10 μm of width) were fabricated using photolithography as previously described67 (link). Briefly, the glass surface was first activated with plasma (Plasma Cleaner, Harrick Plasma) and then coated with cell repellent PLL-g-PEG (Surface Solutions GmbH, 0.5 mg/ml in 10 mM HEPES pH 7.3). After washing with PBS and deionized water, the surface was illuminated with deep UV light (UVO Cleaner, Jelight) through a chromium photomask (JD-Photodata). Finally, coverslips were incubated with fibronectin (Sigma, 25 μg/ml in 100 mM NaHCO3 pH 8.4). Cells were detached using EDTA 0.02% (Versane; GIbco) and left to attach on micropatterns. Afterwards, the coverslips were mounted in 35 mm magnetic chambers (Chamlide) for imaging. Cell locomotion was monitored by using a motorized Olympus Scan^R inverted microscope with ×10 objective for a 10 h period, acquiring images every 5 min. All the experiments were performed using an environmental microscope incubator set to 37 °C and 5% CO2 perfusion.
Corallino S., Malinverno C., Neumann B., Tischer C., Palamidessi A., Frittoli E., Panagiotakopoulou M., Disanza A., Malet-Engra G., Nastaly P., Galli C., Luise C., Bertalot G., Pece S., Di Fiore P.P., Gauthier N., Ferrari A., Maiuri P, & Scita G. (2018). A RAB35-p85/PI3K axis controls oscillatory apical protrusions required for efficient chemotactic migration. Nature Communications, 9, 1475.
+ Open protocol
+ Expand
8

Monitoring Random Cell Motility

Check if the same lab product or an alternative is used in the 5 most similar protocols
The capability of cells in moving in the absence of any chemotactic stimulus was monitored by time-lapse phase contrast microscopy. Cells were seeded sparsely in a six-wells plate (1 × 104 cells/well) in complete media and where indicated, supplemented with doxycycline (2.5 μg/ml) 16 h before starting the experiment. Random cell motility was monitored over a 24 h period. Pictures were acquired every 5 min from 6 positions/condition, using a motorized Olympus Scan^R inverted microscope with ×4 objective. All the experiments were performed using an environmental microscope incubator set to 37 °C and 5% CO2 perfusion. Single cells were manually tracked using Manual Tracking Tool ImageJ software plugin. Migration plot and relative parameters were obtained by Chemotaxis Tool from Ibidi.
Corallino S., Malinverno C., Neumann B., Tischer C., Palamidessi A., Frittoli E., Panagiotakopoulou M., Disanza A., Malet-Engra G., Nastaly P., Galli C., Luise C., Bertalot G., Pece S., Di Fiore P.P., Gauthier N., Ferrari A., Maiuri P, & Scita G. (2018). A RAB35-p85/PI3K axis controls oscillatory apical protrusions required for efficient chemotactic migration. Nature Communications, 9, 1475.
+ Open protocol
+ Expand
9

Measuring Cryptic Lamellipodia Dynamics

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Assays were performed as previously described.14 (link) Briefly, DCIS-RAB5A cells stably expressing EGFP-LifeAct were mixed in a 1:10 ratio with unlabeled DCIS-RAB5A cells and transfected and seeded as described for the wound healing experiment. Cell migration was monitored by time-lapse phase-contrast and fluorescence microscopy with an Olympus ScanR inverted microscope using a 20× objective and images were acquired every 90 s over a 6-h period. The quantification of cryptic lamellipodia protrusion velocity was performed using the ADAPT plug-in of Fiji. Cryptic lamellipodia directionality was measured as the angle Φ delimited by the direction of the single lamellipodium and the direction vector of the collective pack locomotion.
0° ≤ Φ ≤ 45° indicates that protrusion and collective migration have the same direction; Φ = 180° indicates that protrusion and collective migration have opposite directions. The assay was repeated five times for each condition and at least 25 cells/condition were counted for each experiment.
He X, & Lehman I.R. (2001). An initial ATP-independent step in the unwinding of a herpes simplex virus type I origin of replication by a complex of the viral origin-binding protein and single-strand DNA-binding protein. Proceedings of the National Academy of Sciences of the United States of America, 98(6), 3024-3028.
+ Open protocol
+ Expand
10

Wound Healing Assay using Time-Lapse Microscopy

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Assays were performed as previously described.14 (link) Briefly, cells transfected in suspension were seeded at confluency in six-well plates (1.5 × 106 cells/well) in complete medium and transfected again the following day. Three days after seeding, a uniform monolayer is formed. RAB5A, DOCK7 and RAC1 WT/P29S expression was induced 16 h before the experiment was initiated by adding fresh complete media supplemented with 2.5 μg/mL doxycycline to the cells. The cell monolayer was scratched with a pipette tip and carefully washed with PBS to remove floating cells and create a cell-free wound area. The closure of the wound was monitored by time-lapse microscopy. At the time of recording, fresh media containing EGF was added to the cells. The assay was performed using an environmental microscope incubator set to 37°C and 5% CO2 perfusion. An Olympus ScanR inverted microscope with 10× objective was used to acquire images every 5 min over a 24-h period. The percentage of area covered by cells (area coverage %) over time and wound-front speed were calculated using a custom Fiji and MATLAB code. The area covered over time was fitted with a straight line whose slope was used to estimate the velocity of wound closure.
He X, & Lehman I.R. (2001). An initial ATP-independent step in the unwinding of a herpes simplex virus type I origin of replication by a complex of the viral origin-binding protein and single-strand DNA-binding protein. Proceedings of the National Academy of Sciences of the United States of America, 98(6), 3024-3028.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!