Sulfosuccinimidyl 6 3 2 pyridyldithio propionamido hexanoate sulfo lc spdp

Lyophilized fibrinogen from human plasma and Millex syringe filter (0.22 μm) were purchased from EMD Millipore (Billerica, MA). Lyophilized thrombin from bovine plasma, calcium chloride, ε-aminocaproic acid (εACA), and Alcian Blue 8GX were purchased from Sigma-Aldrich (St. Louis, MO). Peptides were synthesized by University of Utah DNA/Peptide synthesis core facility. Spectra/Por 7 dialysis membrane (MWCO = 3.5 kDa) was purchased from Spectrum Laboratories (Rancho Dominguez, CA). Coomassie Brilliant Blue R-250 was purchased from Genlantis (San Diego, CA). Sulfosuccinimidyl 6-(3'-(2-pyridyldithio)propionamido)hexanoate (Sulfo-LC-SPDP) and DyLight 680 NHS-ester were purchased from Thermo Fisher Scientific (Newington, NH). Mini-PROTEAN TGX precast electrophoresis gel was purchased from Bio-Rad (Hercules, CA). TO-PRO-3 iodide, Alexa Fluor 488 conjugated anti-rabbit IgG secondary antibody and Alexa Fluor 568 conjugated anti-mouse IgG secondary antibody were purchased from Invitrogen (Carlsband, CA). Rabbit anti-aquaporin 5 (AQP5), rabbit anti-TMEM-16A, rabbit anti-PECAM-1, mouse anti-cytokeratin 7, mouse anti- β-tubulin III, rabbit anti-Ki67 and Picrosirius Red Stain Kit were purchased from Abcam (Cambridge, MA). Mouse Na⁺/K⁺-ATPase antibody was purchased Santa Cruz Biotechnology (Santa Cruz, CA).

The IgG4 was kindly donated by UCB Celltech (UCB Celltech, Slough, UK). It is a purified IgG4 kappa antibody; the hinge region is not mutated. The IgG was formulated at 17.8 mg/mL in 270 mM glycine, 1% maltose, pH 5.0. This IgG4 kappa has an isoelectric point between 6.85 and 8.15 and a molecular weight of 165 kDa.
Silicon wafers for reflectivity at the NIST Center for Neutron Research (NCNR) were from El-Cat (New Jersey, USA); other NCNR reflectivity cell components were custom made for laminar flow reflectometry.
Silicon wafers and solid-liquid flow cells (SLFCs) for reflectivity at the ISIS Neutron and Muon Source were purpose built. 3-aminopropyltriethoxysilane (APTS) and sulfosuccinimidyl 6-(3′-(2-pyridyldithio)propionamido) hexanoate (sulfo-LC-SPDP) were from Thermo Scientific Pierce (Thermo Fisher Scientific, Leicestershire, UK).
Recombinant staphylococcal protein A was from Repligen (Waltham, MA, USA). Deuterium oxide (99.9 atom % D) and bovine serum albumin (fraction V) were from Sigma-Aldrich (Dorset, UK); PEG₆₀₀₀ was from VWR Chemicals (Leicestershire, UK). Extra dry acetonitrile was from Fisher (Leicestershire, UK).