Lignin peroxidase

Some enzymes were used: laccase (EC 1.10.3.2) from Pleurotus pulmonarius (also formerly known as P. sajor-caju [51 (link)]), horseradish peroxidase (HRP, EC 1.11.1.7, Sigma-Aldrich, Milan, Italy) from Armoracia rusticana, lignin peroxidase (LiP, EC 1.11.1.14, Sigma-Aldrich) from Phanerochaete chrysosporium, and manganese peroxidase (MnP, EC 1.11.1.13, Sigma-Aldrich) from Ph. chrysosporium. When these enzymes were used, the composition of assay mixtures were changed where appropriate with the systematic omission of the biomimetic catalyst as follows: laccase, 23 E.U., 50 mM potassium phosphate buffer (pH 6); HRP, 3 E.U., 50 mM potassium phosphate buffer (pH 7), 8.8 mM H₂O₂; LiP, 0.05 E.U., 50 mM sodium citrate buffer (pH 3), 0.176 mM H₂O₂; MnP, 0.02 E.U., 50 mM sodium citrate buffer (pH 3), 0.176 mM H₂O₂, 2mM Mn(II) acetate, dissolved in 50 mM sodium malonate buffer (pH 4.5).

The following reagents were used in the applied synthetic procedures: tetraethoxysilane (TEOS, Si(OC₂H₅)₄, Sigma-Aldrich Sweden AB, cat. no. 8.00658 98%, Stockholm, Sweden); iron (II) chloride tetrahydrate (FeCl₂·4H₂O, Sigma-Aldrich Sweden AB, cat. no. 380024 97%, Stockholm, Sweden); iron (III) chloride hexahydrate (FeCl₃·6H₂O, Sigma-Aldrich Sweden AB, cat. no. 31232-M 98, Stockholm, Sweden); sodium acetate tri-hydrate (VWR International AB, product: 27652.232, Stockholm, Sweden); glacial acetic acid (Scharlau, HPLC grade, Barcelona, Spain); hydrogen peroxide (H₂O₂, 36% in water, Sigma-Aldrich Sweden AB, Stockholm, Sweden); acetaminophen (Sigma-Aldrich Sweden AB, powder 98.0–102.0%, Stockholm, Sweden); diclofenac sodium salt (Sigma-Aldrich Sweden AB, product number: D6899, Stockholm, Sweden); ethanol (96%); 0.1 M HCl; 0.1 M NaOH; 0.06 M phosphate buffer (pH 7.0); horseradish peroxidase, (Sigma-Aldrich Sweden AB, lyophilized, powder, beige, ~150 U/mg, Stockholm, Sweden); lignin peroxidase (Sigma-Aldrich Sweden AB, powder, slightly beige, >0.1 U/mg, Stockholm, Sweden); ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid), Sigma-Aldrich Sweden AB, Stockholm, Sweden).