Human mesenchymal stem cells (hMSCs) were obtained from Lonza (Walkersville, MD, USA). Cells were cultured and maintained in mesenchymal stem cell growth medium (Lonza, Walkersville, MD, USA) following the manufacturer’s instructions. Cells at passage 5 were used in the present study.
Human mesenchymal stem cells hmscs
Manufactured by Lonza
Sourced in Switzerland
Human mesenchymal stem cells (hMSCs) are multipotent stem cells that can differentiate into various cell types, including osteoblasts, chondrocytes, myocytes, and adipocytes. They are isolated from various tissues such as bone marrow, adipose tissue, and dental pulp.
Automatically generated - may contain errors
Lab products found in correlation
Mesenchymal stem cell growth medium, by Lonza (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Ammonium hydroxide solution, by Merck Group (1 mentions)
Cetylpyridinium chloride, by Merck Group (1 mentions)
Alkaline phosphatase alp staining, by Merck Group (1 mentions)
Pierce bicinchoninic acid bca protein assay kit, by Thermo Fisher Scientific (1 mentions)
L ascorbic acid, by Merck Group (1 mentions)
Alizarin red s, by Merck Group (1 mentions)
β glycerol phosphate, by Merck Group (1 mentions)
Alexa fluor 488 conjugated phalloidin, by Thermo Fisher Scientific (1 mentions)
Dulbecco s phosphate buffered saline dpbs, by Biowest (1 mentions)
Dmem high glucose media, by Biowest (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
6 protocols using human mesenchymal stem cells hmscs
1
Culturing Human Mesenchymal Stem Cells
2
Osteogenic Differentiation of Stem Cells on Titanium
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Example 5
The biomaterial used was titanium Ti6Al4V metal alloy pellets. Human Mesenchymal Stem Cells (hMSCs) were obtained from Lonza®, Inc. Cells were then cultured in low glucose Dulbecco's Modified Eagle Medium (DMEM, Invitrogen®) supplemented with 10% (v/v) fetal bovine serum (FBS), 1% penicillin/streptomycin and incubated in a humidified atmosphere containing 5% (v/v) CO2 at 37° C. All the cells were used at a low passage number (5 passage 3), were subconfluently cultured and were seeded at 10 000 cells/cm2 for the purpose of the experiments. Stem cell phenotype was analyzed after 62 hours of cell culture on titanium covalently modified with GFR-binding compounds as defined herein (SEQ: ID NO: 15 to 20).
To probe this lineage specification, immunostaining for Runx2 and Osterix was performed after cell fixation as described in the Methods section. The quantification of Runx2 and Osterix positive contact numbers and areas was performed as described in the Methods section.
As shown in
3
Osteogenic Differentiation of hMSCs
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F127 powder, dimethyl sulfoxide (DMSO), L-ascorbic acid, β-glycerol phosphate, 3-[4,5-diemthyl-thiazol-2-yl]2,5-diphenyltetrazolium bromide, alizarin red s, cetylpyridinium chloride, ammonium hydroxide solution, and alkaline phosphatase (ALP) staining were all purchased to Sigma Aldrich (St. Louis, MO, USA). Simulated body fluid was purchased to Biosesang Co. (Seongnam-si, Korea). SensoLyte pNPP ALP assay kit was purchased to Anapspec (Fremont, CA, USA). Human mesenchymal stem cells (hMSCs) were purchased from Lonza (Basel, Switzerland). Dulbecco’s modified eagle medium (DMEM, High Glucose, GultaMAXTM), fetal bovine serum (FBS), and 1% antibiotics/antibiotic (AA), and PierceTM Bicinchoninic acid (BCA) Protein Assay kit were all purchased to Thermo Fisher Scientific (Waltham, MA, USA).
4
HA-Based Hydrogels for hMSC Cultivation
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Hyaluronic acid sodium salt (HA) (1 MDa) was purchased from Lifecore (Chaska, MN, USA). The 4‐(4,6‐Dimethoxy1,3,5‐triazin‐2‐yl)−4‐methylmorpholinium chloride (DMTMM), spermidine (SM), (3‐Aminomethylphenyl) boronic acid (PBA), β‐glycerophosphate and dimethyl sulfoxide (DMSO) were purchased from Sigma–Aldrich (St. Louis, MO, USA). Human mesenchymal stem cells (hMSCs) were purchased from Lonza (Basel, Switzerland). DMEM high glucose media and Dulbecco's phosphate‐buffered saline (DPBS) were purchased from Biowest (Riverside, MO, USA). Antibodies and Hoechst were purchased from Invitrogen (Waltham, MA, USA). Sulfo‐cy5.5‐amine was purchased from Lumiprobe (Cockeysville, MD, USA). Basic fibroblast growth factor (bFGF) proteins were purchased from R&D Systems (Minneapolis, MN, USA). Alexa‐Fluor 488‐conjugated phalloidin was purchased from Thermo Fisher Scientific (Waltham, MA, USA). About abbreviations, the unsynthesized HA hydrogel was named free HA (fHA), the HA hydrogel conjugated with PBA was named HAp hydrogel, and the HA hydrogel conjugated with PBA and SM was named HAps. In addition, the samples with growth factors were named fHAG and HApsG, respectively.
5
Isolation and Characterization of hMSCs
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The study was approved by Comité de Bioética at the Universidad Tecnológica de Pereira (Code: 04-180520, 20 May 2020). Human Mesenchymal Stem Cells (hMSCs) were obtained from Lonza Bioscience, Basel, Switzerland, Catalog: PT-2501).
6
Chemical Synthesis and Cell Culture
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Unless otherwise stated, all chemicals for chemical synthesis were of ACS grade, purchased from Sigma-Aldrich and used as received without further purification. For cell culture, all reagents were purchased from Invitrogen (Carlsbad, CA) unless otherwise noted. Human Mesenchymal Stem Cells (HMSCs) were purchased from Lonza (Gaithersburg, MD).
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