Anti p27 sc 528

NSC139021(HY-112158) was purchased from MedChemExpress (Monmouth Junction, NJ, USA) and was solubilized in dimethylsulfoxide (Sigma-Aldrich, St. Louis, MO, USA) at 20 mM and 75 mg/mL stock solution. Antibodies were as follows: anti-Cyclin A (sc-751), anti-CyclinB1 (sc-245), anti-Cyclin E (sc-25303), anti-Skp2 (sc-7164), anti-p-Rb (Ser807) (sc-293117), and anti-p27 (sc-528) were purchased from Santa Cruz Biotechnologies (Dallas, DE, USA); anti-RIOK1 (17222-1-AP), anti-RIOK3 (13593-1-AP), anti-GAPDH (60004-1-Ig), anti-p21 (10355-1-AP), and anti-β-actin (66009-I-Ig) were purchased from Proteintech (Wuhan, China); anti-p53 (2524S), anti-caspase-3 (9662S) were purchased from Cell Signaling Technology (Boston, MA, USA); anti-Bcl-2 (A19693) and anti-Bax (A19684) were purchased from ABclonal (Wuhan, China); anti-RIOK2 (HPA005681) was from Sigma-Aldrich (St. Louis, MO, USA); homemade polyclonal antibody of CDK2 was the gift from Dr. Hui Zhang (Department of Chemistry and Biochemistry, University of Nevada, Las Vegas, NV 89154, USA). Cell Counting Kit-8 (CCK-8) (BA00208) was obtained from Bioss (Beijing, China), and Annexin-V-FITC/PI kit (40302ES60) was purchased from YEASEN (Shanghai, China).