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5 protocols using s 1 phenylethanol

1

Enzymatic Synthesis of Chiral Alcohols

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(R,S)-1-phenylethanol (CAS No. 98-85-1), (R)- and (S)-1-phenylethanol (CAS No.1517-69-7 and 1445-91-6) , 1-phenylethyl acetate (CAS No. 93-92-5), vinyl acetate, hexane and methyl tert butyl ether (MTBE) were purchased from Sigma-Aldrich (Sigma-Aldrich Corp., St. Louis, MO, USA) and Merck KGAA, Darmstadt, Germany. The commercial lipase Novozyme 435 (activity more than 5,000 PLU/g) was kindly donated by Novozymes.
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2

Enantioselective Biocatalytic Reduction

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Acetophenone (ACP), racemic 1-phenylethylamine (1-PEA), (R)-1-PEA, (S)-1-PEA and sodium pyruvate were purchased from Merck (Hohenbrunn, Germany); (R)-1-phenylethanol and (S)-1-phenylethanol from Sigma-Aldrich (Steinheim, Germany); PLP from AppliChem (Darmstadt, Germany); and all other chemicals from VWR (Leuven, Belgium).
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3

Enantiomeric Amino Acid Analysis

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l- and d-enantiomers of methionine (Met), phenylalanine (Phe), and tryptophan (Trp) with ≥98% optical purity were purchased from Sigma-Aldrich Sweden AB (Stockholm, Sweden) and used without further purification. (R)-2-butanol, (S)-2-butanol, a racemic mixture of (R)- and (S)-2-butanol enantiomers, and (S)-1-phenylethanol of 99% purity were obtained from Sigma-Aldrich and used without further purification as target gases. The amino acids were dissolved in a water/methanol/formic acid mixture (49:49:2 v/v/v) at a concentration of 0.04 mM.
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4

Enantioselective Reduction of Acetophenone

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Acetophenone, racemic 1-phenylethylamine (1-PEA), and the pure enantiomers (R)-1-PEA and (S)-1-PEA, all with a purity of ≥98.0 %, were purchased from Merck (Hohenbrunn, Germany). (R)-1-phenylethanol and (S)-1-phenylethanol were purchased from Sigma-Aldrich (Steinheim, Germany), both with a purity of ≥98.0 %, and all other chemicals were from VWR (Leuven, Belgium).
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5

Enzymatic Oxidation of Lignin Derivatives

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Hydrogen peroxide were purchased from Junsei Chemical Co., Ltd. (Tokyo, Japan). Potassium phosphate monobasic and potassium phosphate dibasic were bought from Samchun Chemical Co., Ltd. (Seoul, Korea). Lignosulfonic acid sodium salt, lignin (alkali), peroxidase from horseradish, nitrotetrazolium blue chloride, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, magnesium sulfate, ethyl acetate, 1-octanol, ethylbenzene, (R)-1phenylethanol, (S)-1-phenylethanol, acetophenone, iron(III) chloride hexahydrate, and sodium nitrate were purchased from Sigma-Aldrich (St. Louis, MO, USA). O2 and N2 gases (purity: 99.999%) were bought from Special Gas Co. (Daejeon, Korea). We used type 1 ultrapure water (18 MΩ cm) from a Direct-Q ® 5 UV ultrapure water purification system (Millipore Corp., USA). A recombinant unspecific peroxygenase from Agrocybe aegerita (rAaeUPO) was prepared as described previously 8, 9 .
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