Ab70774

Manufactured by Abcam

Ab70774 is a lab equipment product from Abcam. It is a device used for laboratory analysis and experimentation.

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3 protocols using ab70774

Chromatin Immunoprecipitation Validation Protocol

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ChIP assays were performed with H1299 cells in accordance with
standard protocols^{43 (link)}. Normal rabbit IgG served as the control. ChIP samples were
analyzed by quantitative real-time PCR using the FastStart Universal SYBR Green
Master (Roche). A standard curve was prepared for each set of primers using serial
titration of the input DNA. The percentage of ChIP DNA was calculated relative to
the input DNA from primer-specific standard curves using the Rotor-Gene 6000
Series Software 1.7. The primer sequences for ChIP are listed in Supplementary
Table 4. Antibodies used were: H4S1ph
(Abcam; ab14723), H4K5ac (Millipore; CS204381), H4R3me2a (Active Motif; 39705),
H4R3me2s (Abcam; ab5823), CK2α (Abcam; ab70774), H3K4me3 (Abcam; ab8580), and
H3K27me3 (Abcam; ab6002).

Ju J., Chen A., Deng Y., Liu M., Wang Y., Wang Y., Nie M., Wang C., Ding H., Yao B., Gui T., Li X., Xu Z., Ma C., Song Y., Kvansakul M., Zen K., Zhang C.Y., Luo C., Fang M., Huang D.C., Allis C.D., Tan R., Zeng C.K., Wei J, & Zhao Q. (2017). NatD promotes lung cancer progression by preventing histone H4 serine phosphorylation to activate Slug expression. Nature Communications, 8, 928.

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Antibody Detection in Cell Cultures

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Antibodies used in the experiments were obtained from the following companies: rabbit monoclonal anti‐CK2β antibody (1:1000) (ABGENT; AJ1128b); mouse monoclonal anti‐β‐ACTIN antibody (1:1000) (Easybio Technology; BE0021); mouse monoclonal anti‐MVH antibody (1:200) (Abcam; ab27591); mouse monoclonal anti‐CK2α antibody (1:1000) (Abcam; ab70774); and rabbit polyclonal anti‐CK2α′ antibody (1:1000) (Bioworld Technology; BS6571); secondary antibodies were purchased from Zhongshan Golden Bridge Biotechnology Co, LTD.

Liang Q., Wang Z., Lei W., Lin F., Qiao J., Filhol‐Cochet O., Boldyreff B., Schatten H., Sun Q, & Qian W. (2019). Deletion of Ck2β gene causes germ cell development arrest and azoospermia in male mice. Cell Proliferation, 53(1), e12726.

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Antibody Usage in Protein Analysis

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The following antibodies were used for immunoblotting and immunoprecipitation: polyclonal anti-ZC3H4 antibody, developed against a purified GST-tagged human ZC3H4 fragment (amino acid residues 1114–1315) in this study; polyclonal anti-WDR82 antibody (kindly provided by the Roeder laboratory); anti-CK2α (10992-1-AP, Proteintech); anti-CK2β (ab76025, Abcam); anti-SPT5 (A300-869A for immunoblotting; A300-868A for immunoprecipitation, Bethyl Laboratories); anti-RNAPII (ab26721, Abcam); anti-S5p RNAPII (ab5131, Abcam); anti-S2p RNAPII (ab5095, Abcam); anti-β-actin (TA811000, Origene); anti-phospho-CK2 substrate [(pS/pT)DXE] (8738, Cell Signaling Technology) and anti-FLAG (A8592, Sigma-Aldrich). The following antibodies were used for ChIP-seq analyses: anti-ZC3H4 (this study) and anti-WDR82 (48 (link)), generated in house; anti-CK2α (ab70774, Abcam); anti-SPT5 (sc-133217, Santa Cruz); anti-RNAPII (A300-653A, Bethyl Laboratories); anti-S5p RNAPII (ab5131, Abcam); anti-Sp2 RNAPII (A300-654A, Bethyl Laboratories); anti-H3K4me3 (C15410003, Diagenode); anti-H3K27me3 (9733, Cell Signaling Technology) and anti-H3K36me3 (61101, Active Motif).

Park K., Zhong J., Jang J.S., Kim J., Kim H.J., Lee J.H, & Kim J. (2022). ZWC complex-mediated SPT5 phosphorylation suppresses divergent antisense RNA transcription at active gene promoters. Nucleic Acids Research, 50(7), 3835-3851.

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