Human umbilical vein endothelial cells (huvec)

Manufactured by Shanghai Cell Bank

Sourced in China

HUVEC is a type of primary human endothelial cell derived from the umbilical vein. It is commonly used in in vitro studies to model human endothelial cell function and behavior.

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Lab products found in correlation

7 protocols using human umbilical vein endothelial cells (huvec)

Culture and Maintenance of Cell Lines

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The immortalized human EVT cell line HTR-8/SVneo and the human choriocarcinoma cell line JEG-3 were provided by Zhou Chen from State Key Laboratory. DMEM/F-12 and RPMI (Gibco, Waltham, MA, USA) were used to culture HTR-8/SVneo and JEG-3, respectively, supplemented with 12% fetal bovine serum (FBS). Furthermore, the human umbilical vein endothelial cell line Huvec was obtained from Shanghai Cell Bank (Beyotime Biotechnology, Shanghai, China) and cultured in a DMEM medium. All cells were cultured in a humidified atmosphere with 5% CO₂ at 37 °C.

Ramírez-Coronel A.A., Rostami A., Younus L.A., Arias Gonzáles J.L., Lafta M.H., Amin A.H., Saadoon M.A., Salman H.M., Bahrami A., Feilei R, & Akhavan-Sigari R. (2023). Designing Effective Multi-Target Drugs and Identifying Biomarkers in Recurrent Pregnancy Loss (RPL) Using In Vivo, In Vitro, and In Silico Approaches. Biomedicines, 11(3), 879.

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Ovarian Cancer Cell Line Cultivation

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The A2780, SKOV3, and OVCA429 human ovarian cancer cell lines, the ID8 mouse ovarian cancer cell line, the IOSE80 normal ovarian epithelial cell line, and the HUVEC human umbilical vein endothelial cell line were obtained from Shanghai Cell Bank and Professor Tsz on Lee University of Macau. The cell lines were cultured in DMEM supplemented with 10% certified fetal bovine serum and 1% penicillin–streptomycin and maintained in a humidified incubator at 37 °C with 5% CO2.

Li N., Jiang X., Ma X., Qiu X., Chang H., Qiao Y., Luo H, & Zhang Q. (2023). Antimicrobial peptides CS-piscidin-induced cell death involves activation of RIPK1/PARP, and modification with myristic acid enhances its stability and tumor-targeting capability. Discover. Oncology, 14, 38.

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Culturing Immortalized Human Cell Lines

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The immortalised human EVT cell line HTR-8/SVneo and the human choriocarcinoma cell line JEG-3 was kindly provided by Chao Tong from State Key Laboratory of Maternal and Foetal Medicine of Chongqing Municipality. HTR-8/SVneo and JEG-3 were cultured in RPMI 1640 (Gibco, Waltham, MA, USA) and DMEM/F-12 medium (Gibco), respectively, supplemented with 10% foetal bovine serum (FBS) (PAN). Furthermore, the human umbilical vein endothelial cell line Huvec was obtained from Shanghai Cell Bank (Shanghai, China) and cultured in DMEM medium (Gibco). All cells were cultured in a humidified atmosphere with 5% CO₂ at 37 °C.

Duan Y., Fu H., Huang J., Yin N., Liu L, & Liu X. (2022). TOP2A deficiency leads to human recurrent spontaneous abortion and growth retardation of mouse pre-implantation embryos. Molecular Medicine, 28, 165.

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Hepatoma Cell Characterization Protocol

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Human hepatoma cell lines,
HUH-7 and Hep3B, and human normal cell lines, HUVEC, BEAS-2B, and
QSG-7701, were obtained from Shanghai Cell Bank of Chinese Academy
of Sciences, China; RPMI-1640 culture medium, fetal bovine serum,
and trypsin were obtained from Thermo Fisher Scientific (China) Co.,
Ltd; MLs, DAPI, CK8/18/19-FITC,^{24 (link),25 (link)} CD45-PE, and magnetic
separation rack were obtained from Huzhou Lieyuan Medical Laboratory
Co., Ltd; anti-GPC3 and anti-EpCAM antibodies were obtained from Abcam;
the Prussian Blue Iron Stain Kit (Nuclear fast red) was obtained from
Beijing Solarbio Science & Technology Co., Ltd; distearoylphosphatidylethanolamine-polyethylene
glycol (DSPE-PEG), 1,2-dioleoyl-sn-glycero-3-phosphocholine
(DOPC), hexadecyl-quaternized (HQCMC), glycidyl hexadecyl dimethylammonium
chloride (GHDC), N-hydroxysuccinimide (NHS), 1-ethyl-3-(3-dimethylaminopropyl)
carbodiimide hydrochloride (EDC·HCL), and agarose were obtained
from JuKang (Shanghai) Biotechnology Co., Ltd; cholesterol, hydrochloric
acid, and other common reagents were obtained from Sinopharm Chemical
Reagent Co., Ltd; and the cell proliferation and toxicity test kit
(CCK-8) was obtained from Shanghai Yisheng Biology Co., Ltd.

Huang Z., Li F., Zhang J., Shi X., Xu Y, & Huang X. (2022). Research on the Construction of Bispecific-Targeted Sustained-Release Drug-Delivery Microspheres and Their Function in Treatment of Hepatocellular Carcinoma. ACS Omega, 7(25), 22003-22014.

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Culturing Cancer and Endothelial Cells

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Mouse liver cancer cells (Hepa 1-6), human breast cancer cells (MCF-7), and human umbilical vein endothelial cells (HUVEC) were purchased from Cell Bank of Shanghai, Chinese Academy of Science (CAS, Shanghai, China). The cells were cultured in high-glycemic Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin and streptomycin. Cells were cultured in an incubator (Esco Micro Pte. Ltd., Changi, Singapore) in an atmosphere of 5% CO₂ and 90% relative humidity at 37°. In order to maintain good growth conditions, culture operations were carried out in accordance with the ATCC’s instructions. The daily growth status of the cells was observed under a binocular phase contrast microscope, and the cells growing in the logarithmic phase were used in related experiments.

Lv Y., Chen X., Chen Z., Shang Z., Li Y., Xu W., Mo Y., Wang X., Xu D., Li S., Wang Z., Wu M, & Wang J. (2022). Melittin Tryptophan Substitution with a Fluorescent Amino Acid Reveals the Structural Basis of Selective Antitumor Effect and Subcellular Localization in Tumor Cells. Toxins, 14(7), 428.

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Lung Cancer Cell Line Characterization

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Human lung cancer cell lines (A549, H1975, H460, H446) and human umbilical vein endothelial cell line (HUVEC) were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences. Fe₃O₄ solution, carboxymethyl chitosan hexadecyl quaternary ammonium salt (HQCMC), 1,2-dioleoylphosphatidylcholine (DOPC), and dimethyloctadecylglycidyl ammonium chloride (GHDC) were purchased from Huzhou Lieyuan Medical Laboratory Co, Ltd dulbecco’s modification of eagle’s medium dulbecco (DMEM)/1640 medium, fetal bovine serum, and trypsin were purchased from Gibco. Epithelial cell adhesion molecule, vimentin, and EGFR antibodies were purchased from eBioscience. Cholesterol, dichloromethane, N-hydroxysuccinimide (NHS), 1-ethyl-3-(3-dimethylammoniumpropyl) ammonium carbonate (EDC), and other common reagents were purchased from Sinopharm Group. Prussian blue staining kit was purchased from Solarbio.

Jiang W., Wu J., Lin X., Chen Z., Lin L, & Yang J. (2024). Enumeration and Molecular Characterization of Circulating Tumor Cell Using an Epithelial Cell Adhesion Molecule/Vimentin/Epidermal Growth Factor Receptor Joint Capture System in Lung Cancer. Clinical Medicine Insights. Oncology, 18, 11795549241231568.

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Isolation and Polarization of Macrophages

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HUVEC was purchased from Shanghai Cell Bank (Chinese Academy of Sciences, Shanghai, China). The authentication of cells by Short Tandem Repeat (STR) DNA profiling has been done. Monthly mycoplasma testing to confirm that cells were not contaminated with mycoplasma. A complete endothelial cell medium (ScienCell, CA, United States) was used for HUVECs in vitro. Obtain monocyte-derived macrophages (MDM) according to the previous protocol (Menck et al., 2014 (link); Kelly et al., 2018 (link)). Briefly, peripheral blood mononuclear cells (PBMC) were purified from human peripheral blood buffy coats by two gradient centrifugations, Mature MDM (M₀) was then obtained by culturing in 1640 complete medium (BasalMedia, Shanghai, China) containing 50 ng/ml M-CSF for 7 days. Finally, M2 was obtained by polarizing M0 with 20 ng/ml of IL-4 for 48 h. M-CSF (# 300-25) and IL-4 (# 200-04) were purchased from PeproTech (NJ, United States).

Lyu L., Cai Y., Zhang G., Jing Z., Liang J., Zhang R., Dang X, & Zhang C. (2022). Exosomes derived from M2 macrophages induce angiogenesis to promote wound healing. Frontiers in Molecular Biosciences, 9, 1008802.

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