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Maxwell 16 research extraction system

Manufactured by Promega
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The Maxwell 16 Research Extraction System is an automated instrument designed for nucleic acid extraction. It utilizes magnetic particle separation technology to efficiently purify DNA, RNA, or other biomolecules from a variety of sample types. The system provides consistent and reliable results, streamlining the extraction process to save time and improve workflow efficiency in research laboratories.

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Lab products found in correlation

Maxwell 16 ffpe plus lev rna purification kit, by Promega (2 mentions) Turbo dna free kit, by Thermo Fisher Scientific (1 mentions) Maxwell rsc rna ffpe kit, by Promega (1 mentions) Qubit 2.0 fluorometer, by Thermo Fisher Scientific (1 mentions) Miseq platform, by Illumina (1 mentions) Agilent bioanalyzer, by Agilent Technologies (1 mentions) Trusight rna fusion panel, by Illumina (1 mentions) Qubit fluorometer, by Thermo Fisher Scientific (1 mentions)

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Maxwell 16 (79 citations) Maxwell 16 system (66 citations) Maxwell system (24 citations) MaxWell 16 research system (15 citations) Maxwell 16 Research (11 citations)

4 protocols using maxwell 16 research extraction system

1

RNA Extraction and Sequencing Workflow

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RNA extraction, library preparation, and semiconductor sequencing were performed as described previously [25 (link),33 (link)]. A summary of the entire workflow is depicted in Figure 1. In short, tumor tissue was macrodissected to achieve a histological tumor cell content of at least 15%. The extraction was carried out with the automated Maxwell® 16 Research extraction system (Promega, Madison, WI, USA) and the Maxwell® 16 FFPE Plus LEV RNA Purification Kit following the manufacturer’s instructions. The samples were then digested with the TURBO DNA-free ™ Kit (Thermo Fisher Scientific, Waltham, MA, USA) to obtain DNA-free RNA. The concentration of RNA was measured fluorimetrically (QuBit 2.0 RNA high sensitivity kit (Thermo Fisher Scientific).
Kirchner M., Neumann O., Volckmar A.L., Stögbauer F., Allgäuer M., Kazdal D., Budczies J., Rempel E., Brandt R., Talla S.B., von Winterfeld M., Leichsenring J., Bochtler T., Krämer A., Springfeld C., Schirmacher P., Penzel R., Endris V, & Stenzinger A. (2019). RNA-Based Detection of Gene Fusions in Formalin-Fixed and Paraffin-Embedded Solid Cancer Samples. Cancers, 11(9), 1309.
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2

Extracting Tumor Nucleic Acids from FFPE Tissue

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Tumor DNA and RNA were extracted from 5μm-thick formalin-fixed paraffin-embedded (FFPE) tissue sections based on annotation of a corresponding H&E slide by a pathologist. Samples from either radical cystectomy specimens with muscle invasive bladder cancer or transuretheral resection of bladder tumors (TURBT) were used. Areas were chosen to ensure a 70% minimum tumor cell proportion within the selected region. Extraction was performed by using the Maxwell RSC RNA FFPE kit to isolate total nucleic acids on an automated Maxwell 16 Research extraction system (Promega, Madison, USA). The Maxwell RNase A and DNase I solutions were used to isolate RNA and DNA, respectively, during the two different procedures of nucleic acids isolation. Nucleic acid quantification was performed by the Qubit 2.0 Fluorometer (Thermo Fisher Scientific).
Al-ezzi E.M., Veitch Z.W., Salah S.H., Van der Kwast T.H., Stockley T.L., Selvarajah S., Zhang T., Sridhar S.S., Sacher A.G., Fallah-rad N., Kulkarni G.S., Zlotta A.R., Finelli A, & Hansen A.R. (2021). Genomic characterization of non-schistosomiasis-related squamous cell carcinoma of the urinary bladder: A retrospective exploratory study. PLoS ONE, 16(12), e0259272.
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3

RNA Sequencing of FFPE Tissue

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Two cases were analyzed by targeted RNA sequencing, using RNA extracted from FFPE tissue (cases #1,9). In the index case #1, the RNA was extracted with the Amsbio’s ExpressArt FFPE Clear RNA Ready kit (Amsbio LLC, Cambridge, MA) and the fragment length was assessed with an RNA 6000 chip on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA). RNA-seq libraries were prepared using 20 to 100 ng total RNA with the TruSight RNA Fusion Panel (Illumina, San Diego, CA). Targeted RNA sequencing was performed on an Illumina MiSeq platform. For case # 9, RNA extracted by the automated Maxwell 16 Research extraction system (Promega, Madison, WI, USA) was analyzed on an Ion Torrent S5 sequencing platform using the Archer FusionPlex Sarcoma Assay4 . Reads were independently aligned with STAR (version 2.3) against the human reference genome (hg19) and analyzed by STAR-Fusion.
Antonescu C.R., Dickson B.C., Sung Y.S., Zhang L., Suurmeijer A.J., Stenzinger A., Mechtersheimer G, & Fletcher C.D. (2020). Recurrent YAP1 and MAML2 Gene Rearrangements in Retiform and Composite Hemangioendothelioma. The American journal of surgical pathology, 44(12), 1677-1684.
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4

RNA Extraction from FFPE Samples

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One H&E-stained slide from formalin-fixed paraffin-embedded tissue (FFPE) was obtained for each sample and reviewed by an expert pathologist to evaluate tumor cellularity, which was always greater than 15%. RNA was then isolated from 8 consecutive 10 µm unstained slides, using the automated Maxwell®16 research extraction system (Promega, Madison, WI, USA) and the Maxwell®16 FFPE Plus LEV RNA Purification Kit following the manufacturer’s instructions, and was stored at −80 °C. An RNA concentration evaluation was performed using the Qubit fluorometer (Invitrogen, Carlsbad, CA, USA).
Levacher C., Viennot M., Drouet A., Beaussire L., Coutant S., Théry J.C., Baert-Desurmont S., Laé M., Ruminy P, & Houdayer C. (2023). Disequilibrium between BRCA1 and BRCA2 Circular and Messenger RNAs Plays a Role in Breast Cancer. Cancers, 15(7), 2176.
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