Anti naf1

Manufactured by Abcam

Anti-NAF1 is a primary antibody that detects the Nuclear Assembly Factor 1 (NAF1) protein. NAF1 is involved in the biogenesis and assembly of small nucleolar ribonucleoproteins (snoRNPs), which are required for the processing and modification of ribosomal RNA.

Automatically generated - may contain errors

Lab products found in correlation

Ab157106, by Proteintech (2 mentions) Anti nhp2, by Proteintech (2 mentions) Anti nop10, by Abcam (2 mentions) Anti dyskerin, by Santa Cruz Biotechnology (2 mentions) Anti flag, by Proteintech (2 mentions) Nb100 68252, by Abcam (1 mentions) Anti htert, by Santa Cruz Biotechnology (1 mentions) Anti tip60, by Santa Cruz Biotechnology (1 mentions) Mab1501, by Merck Group (1 mentions) Anti ha, by Cell Signaling Technology (1 mentions) Anti actin, by Merck Group (1 mentions) Anti α tubulin, by Merck Group (1 mentions) Anti his, by Santa Cruz Biotechnology (1 mentions) Anti gar1, by Proteintech (1 mentions)

2 protocols using anti naf1

Protein Expression and IP Analysis

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Analysis of protein expression and IP experiments was performed by resolving proteins by SDS-PAGE, transfer to PVDF and immunoblotting. Primary antibodies used for immunoblotting were: anti-FLAG (Proteintech, 20543-1-AP, 1:4000), anti-HA (Cell Signaling, 2367, 6E2, 1:1125), anti-NAF1 (Abcam, ab157106, 1:1000), anti-NHP2 (Proteintech, 15128–1-AP, 1:5000), anti-NOP10 (Abcam, ab134902, 1:500), anti-TCAB1 (Novus, NB100–68252, 1:2000), anti-reptin (Abcam, ab51500, 1:5000), anti-hTERT (Santa Cruz, sc7215, C-20, 1:500), anti-dyskerin (Santa Cruz, sc-373956, H-3, 1:1500), anti-PARN (Abcam, ab154214, 1:500), anti-RRP40 (Bethyl, A303–909A-T, 1/1500), anti-TIP60 (Santa Cruz, sc5725, N-17, 1/1000), anti-hGAR1 (from Dr Witold Filipowicz (40 (link)), 1/2000) and anti-actin (Chemicon MAB1501, 1:5000).

MacNeil D.E., Lambert-Lanteigne P, & Autexier C. (2019). N-terminal residues of human dyskerin are required for interactions with telomerase RNA that prevent RNA degradation. Nucleic Acids Research, 47(10), 5368-5380.

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Western Blot Analysis of Protein Complexes

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Analysis of protein expression and IP experiments was performed by resolving proteins by SDS-PAGE, transfer to PVDF and immunoblotting. Primary antibodies used for immunoblotting were: anti-FLAG (Proteintech, 20543-1-AP, 1:4000) or anti-FLAG (Proteintech, 66008-3-Ig, 1:4000), anti-NAF1 (Abcam, ab157106, 1:1000), anti-NHP2 (Proteintech, 15128-1-AP, 1:5000), anti-NOP10 (Abcam, ab134902, 1:500), anti-dyskerin (Santa Cruz, sc-373956, H-3, 1:1500), anti-GAR1 (Proteintech, 11711-1-AP, 1:1000), anti-His (Santa Cruz, sc-8036, H-3, 1:500), and anti-alpha tubulin (Sigma, T5168, 1:5000).

MacNeil D., Lambert-Lanteigne P., Jian Q., McManus F.P., Bonneil É., Thibault P., & Autexier C. (2020). SUMOylation- and GAR1-dependent regulation of dyskerin nuclear and subnuclear localization.

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