4 hydroxy flurbiprofen

Phenacetin, acetaminophen, 2-acetamidophenol, coumarin, 7-hydroxy-comuarin (umbelliferone), flurbiprofen, dextromethorphan, dextrorphan, trazodone, thymol, bupropion, 6-hydroxy-bupropion, alprazolam, 1′-hydroxy-alprazolam, NADP⁺, isocitrate dehydrogenase, and DL-isocitrate were purchased from Sigma-Aldrich (St. Louis, MO, USA). Pronethalol was from Tocris (Minneapolis, MN, USA). Propofol was provided by Zeneca Pharmaceuticals (Wilmington, DE, USA). 4′-hydroxy-flurbiprofen, 2-fluoro-4-biphenyl-acetic acid, 4-hydroxy-propofol, chlorzoxazone, and 6-hydroxy-chlorzoxazone were purchased from Toronto Research Chemicals (Toronto, ON, Canada). S-Mephenytoin and 4-hydroxymephenytoin were purchased from Gentest (Corning, Corning, NY, USA). GW340416A, a chemical analogue of bupropion, was kindly provided by GlaxoSmithKline (Research Triangle Park, NC, USA). Omeprazole was purchased from BeanTown Chemical, Inc. (Hudson, NH, USA) and omeprazole sulfone was purchased from Cayman Chemical Company (Ann Arbor, MI, USA). Sodium hydroxide, potassium phosphate monobasic and potassium phosphate dibasic and EDTA were purchased from J.T. Baker (Center Valley, PA, USA). HPLC-grade acetonitrile and methanol were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Ultra-pure water was obtained using a Milli-Q^® Q-POD Millipore System (EMD Millipore, Burlington, MA, USA).

Tunicamycin, 4-phenylbutylate, cycloheximide, ibuprofen, and lysozyme were obtained from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). The ALDH2 protein (18–517 aa) was obtained from ATGen (Seoul, Korea). (±)-Flurbiprofen were from Sigma (St Louis, MO, USA) or Cayman Chemical (Ann Arbor, MI, USA). α-Lactalbumin, aspirin, and meloxicam were from Sigma. Human recombinant leptin for use in vitro was from Sigma and mouse recombinant leptin for use in vivo was from R'D Systems (Minneapolis, MN, USA). Ferriteglycidyl methacrylate (FG) beads (epoxy beads: TAS8848N1110) were from Tamagawa Seiki (Tokyo, Japan). 4′-hydroxy flurbiprofen was obtained from Toronto Research Chemicals (Toronto, ON, Canada).