Newborn calf serum

Manufactured by Harvard Bioscience

Sourced in Germany

Newborn calf serum is a biological supplement derived from the blood of newborn calves. It provides a complex mixture of proteins, growth factors, and other biomolecules that support cell growth and proliferation in cell culture applications.

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Lab products found in correlation

Ethylene diamine tetraacetic acid (edta), by AppliChem (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Montelukast, by Cayman Chemical (1 mentions) Pioglitazone, by Cayman Chemical (1 mentions) Zafirlukast, by Cayman Chemical (1 mentions) Sodium pyruvate, by Thermo Fisher Scientific (1 mentions) 14 15 eet d11, by Cayman Chemical (1 mentions) 3 isobutyl 1 methyl xanthine (ibmx), by Cayman Chemical (1 mentions) Rosiglitazone, by Cayman Chemical (1 mentions) Np 40, by AppliChem (1 mentions) Sodium dodecyl sulfate (sds), by AppliChem (1 mentions) Steponeplus thermocycler, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) Opti mem, by Thermo Fisher Scientific (1 mentions) Oil red o, by Merck Group (1 mentions) Insulin, by Merck Group (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions) Fetal calf serum (fcs), by Capricorn (1 mentions) Sodium chloride (nacl), by AppliChem (1 mentions)

5 protocols using newborn calf serum

Human Corneal Tissue Characterization

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Human corneal donor buttons, unsuitable for transplantation, were obtained from the Lions Cornea Bank Saar-Lor-Lux, Trier/Westpfalz at the Department of Ophthalmology, Saarland University Medical Center, Homburg/Saar, Germany. A total of 39 donor buttons with average storage times of (17 ± 14) weeks were used. From stroma to endothelium, the samples were on average 962 μm thick, with thicknesses varying between approximately 675 and 1190 μm. No significant changes were observed during the experiment duration (thickness changes below 10% in 144 h). Thickness data were obtained using multiphoton tomography. Prior to the experiments, samples were stored in culture Medium II with Dextran T500 (#F9017, Biochrom GmbH, Berlin, Germany) supplemented with 5% new-born calf serum (#S0415, Biochrom GmbH, Berlin, Germany) at 34 °C in a 5% CO₂ atmosphere.
This study was approved by the ethics committee of the University of Saarland and it was conducted according to the principles for research use of human tissue of the World Medical Association Declaration of Helsinki. Written and informed consent for the use of their tissue for scientific research was obtained for all subjects.

Batista A., Breunig H.G., Hager T., Seitz B, & König K. (2019). Early evaluation of corneal collagen crosslinking in ex-vivo human corneas using two-photon imaging. Scientific Reports, 9, 10241.

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3T3-L1 Cell Differentiation Assay

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DMEM, OptiMEM, penicillin-streptomycin, PBS and sodium pyruvate were obtained from Gibco by Life Technologies (Carlsbad, CA, United States), SYBR green MicroAmp Fast Optical 96-well Reaction Plate 0.1 ml and StepOnePlus thermocycler were obtained from Applied Biosystems (Foster City, CA, United States). FCS was purchased from Capricorn (Ebsdorfergrund, Germany). New born calf serum was purchased from Biochrom (Berlin, Germany). All primer pairs were purchased from Eurofins (Friedrichsdorf, Germany). Cell culture flasks (T75, Cell+, vented cap) and 6-well plates for sub-culturing 3T3-L1 cells were obtained from SARSTEDT (Nümbrecht, Germany). Rosiglitazone, pioglitazone, zafirlukast, montelukast, IBMX and (±)14(15)-EET-d₁₁ were purchased from Cayman Chemical (Ann Arbor, MI, United States). Insulin, dexamethasone and Oil Red O were obtained from Sigma Aldrich (St. Louis, Missouri, MO, United States). Tris, Triton-X-100, NP-40, NaCl, EDTA and SDS were purchased from AppliChem (Darmstadt, Germany).

Göbel T., Diehl O., Heering J., Merk D., Angioni C., Wittmann S.K., Buscato E.L., Kottke R., Weizel L., Schader T., Maier T.J., Geisslinger G., Schubert-Zsilavecz M., Steinhilber D., Proschak E, & Kahnt A.S. (2019). Zafirlukast Is a Dual Modulator of Human Soluble Epoxide Hydrolase and Peroxisome Proliferator-Activated Receptor γ. Frontiers in Pharmacology, 10, 263.

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Knee Wear Simulator Protocol

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Before wear testing the gliding surfaces were preconditioned in the test solution until no increase of weight was measurable. The test fluid simulated synovial liquid with a protein content of 30 g/L. The applied lubricant was changed every six days (25% (v/v) newborn calf serum (Biochrom, Germany) with 0.1% (m/v) sodium azide solution in sterile water with EDTA (AppliChem, Germany) for pH stability and Amphotericin B (Biochrom, Germany) as a fungicidal). The lubricant was changed every 0.5 million cycles. The specimens were tested on a servohydraulic knee wear simulator (EndoLab, Germany) with four test stations; the test specifications followed the ISO [15 ].

Paulus A.C., Franke M., Kraxenberger M., Schröder C., Jansson V, & Utzschneider S. (2015). PMMA Third-Body Wear after Unicondylar Knee Arthroplasty Decuples the UHMWPE Wear Particle Generation In Vitro. BioMed Research International, 2015, 575849.

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Cell Line Maintenance and Transfection

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Two sacoma cell lines, HT1080 and SW1353, were maintained in Minimum Essential Medium (MEM; Gibco) and L-15 medium (Leibovitz), respectively. Both medium were supplemented with 10% fetal bovine serum (Biological Industries). HEK293T cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM; Gibco) supplement with 10% newborn calf serum (Biochrom).
Plasmid transfection was carried out by polyethylenimine (PEI; Polysciences) method, according to the manufacturer's protocol.
Cells stably expressing the indicated proteins were established by standard retroviral infection, and selected in 2 μg/ml puromycin (Ameresco) or 50 μg/ml hygromycin B (Ameresco) for 7 days.

Ma S., Jiang B., Deng W., Gu Z.K., Wu F.Z., Li T., Xia Y., Yang H., Ye D., Xiong Y, & Guan K.L. (2015). D-2-hydroxyglutarate is essential for maintaining oncogenic property of mutant IDH-containing cancer cells but dispensable for cell growth. Oncotarget, 6(11), 8606-8620.

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Imaging Human Corneal Endothelium and Descemet's Membrane

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Human corneas were provided by the Lions Cornea Bank Saar-Lor-Lux, Trier/Westpfalz at the Department of Ophthalmology, Saarland University Medical Center, Homburg/Saar, Germany. Until image acquisition, the corneas were stored in Culture Medium II with Dextran T500 (#F9017; Biochrom GmbH, Berlin, Germany) supplemented with 5% newborn calf serum (#S0415; Biochrom GmbH) at 378C under a 5% CO 2 atmosphere.
A total of 10 samples was used. Based on storage time, they were divided into three groups: Short-term storage (STS): <1 week of storage (n ¼ 4) Medium-term storage (MTS): between 3 and 4 weeks of storage (n ¼ 3) Long-term storage (LTS): between 8 and 13 weeks of storage (n ¼ 3) Samples had ECDs of 1733 6 54 and 1824 6 53 cells/mm 2 when calculated manually and with the NAVIS cell count system (Nidek Technologies, Erlangen, Germany), respectively. ECDs were determined in the central region of the cornea.
For image acquisition, the samples were washed in PBS and placed in glass-bottom petri dishes. Live-cell imaging solution (#A14291DJ; Life Technologies, Carlsbad, CA, USA) was used to ensure the cornea hydration during imaging. For imaging the endothelium and the Descemet's membrane, the samples were flipped.
All experiments were conducted following the tenets of the World Medical Association Declaration of Helsinki for research using human samples.

Batista A., Breunig H.G., König A., Schindele A., Hager T., Seitz B., Morgado A.M, & König K. (2018). Assessment of Human Corneas Prior to Transplantation Using High-Resolution Two-Photon Imaging. Investigative ophthalmology & visual science, 59(1).

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