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Mouse epo elisa kit

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The Mouse EPO ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of mouse erythropoietin (EPO) levels in biological samples. It utilizes a specific antibody coated on a microplate to capture EPO proteins present in the sample, which are then detected using a biotinylated detection antibody and streptavidin-conjugated enzyme. The assay provides a reliable and sensitive method for quantifying EPO levels in mouse samples.

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Mouse fibronectin elisa kit, by Assaypro (2 mentions) Mouse icam 1 elisa kit, by R&D Systems (2 mentions) Human kallistatin elisa kit, by R&D Systems (2 mentions) Mouse kallistatin serpina3c, by R&D Systems (2 mentions) Tβr 1 and tβr 2, by Novus Biologicals (2 mentions) Tnf α, by Cell Signaling Technology (2 mentions) Hif 1α, by Novus Biologicals (2 mentions) β actin, by Merck Group (2 mentions) Tgf β1, by R&D Systems (2 mentions) Elisa kit, by Enzo Life Sciences (2 mentions) C57bl 6 male mice, by Inotiv (1 mentions) Microhematocrit capillary tube, by Thermo Fisher Scientific (1 mentions) Haematokrit 210, by Hettich (1 mentions)

9 protocols using mouse epo elisa kit

1

Hematocrit and Cytokine Measurement

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Peripheral blood samples were collected and centrifuged at 10,000g for 5 min to determine hematocrit values and the sera were separated by centrifugation at 1,000g for 15 min. To measure the serum EPO and G-CSF levels, we used an EPO Mouse ELISA Kit and a G-CSF Mouse ELISA Kit (R & D Systems, Minneapolis, MN, USA), according to the manufacturer’s protocols and whole blood was prepared as a blood smear and stained using the May-Grünwald Giemsa staining technique.
Otsuka H., Yagi H., Endo Y., Soeta S., Nonaka N, & Nakamura M. (2016). Nitrogen-containing bisphosphonate induces a newly discovered hematopoietic structure in the omentum of an anemic mouse model by stimulating G-CSF production. Cell and Tissue Research, 367(2), 297-309.
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2

Serum EPO Determination and Blood Smear

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Peripheral blood samples were collected and centrifuged (10000 × g) to determine hematocrit values, and serum was then separated using centrifugation (1000 × g). To obtain serum EPO measurements, we used an EPO Mouse ELISA Kit (R & D systems, Minneapolis, MN, USA) according manufacturer’s protocol, and whole blood was prepared in blood smears and performed the May-Grünwald Giemsa staining technique.
Otsuka H., Takito J., Endo Y., Yagi H., Soeta S., Yanagisawa N., Nonaka N, & Nakamura M. (2016). The expression of embryonic globin mRNA in a severely anemic mouse model induced by treatment with nitrogen-containing bisphosphonate. BMC Hematology, 16, 4.
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3

EPO Quantification in Mouse Plasma

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Eight-week-old C57BL/6 male mice (Envigo, Barcelona, Spain) were dosed intraperitoneally (i.p.) with VCE-004.8 (10 mg/kg) for 3 weeks. Blood samples were taken under general anesthesia, and heparin plasma was collected. Samples were centrifuged for 20 min at 2000×g within 30 min of collection, and circulating levels of plasma EPO were quantified with a mouse EPO ELISA kit (R&D Systems) according to the manufacturer’s instructions. EPO values represent the mean ± SEM.
Navarrete C., Carrillo-Salinas F., Palomares B., Mecha M., Jiménez-Jiménez C., Mestre L., Feliú A., Bellido M.L., Fiebich B.L., Appendino G., Calzado M.A., Guaza C, & Muñoz E. (2018). Hypoxia mimetic activity of VCE-004.8, a cannabidiol quinone derivative: implications for multiple sclerosis therapy. Journal of Neuroinflammation, 15, 64.
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4

Plasma EPO Concentration Measurement

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Peripheral blood was placed into heparinized Micro-Hematocrit Capillary tubes (Thermo Fisher Scientific, Waltham, MA, USA) and centrifuged at 3000 rpm for 20 min at room temperature. After determining the hematocrit value, the plasma was collected for measurement of the plasma EPO concentration. The plasma EPO concentration was measured using a mouse EPO ELISA kit as described in the manufacturer's protocol (R&D Systems, Minneapolis, MN, USA).
Sato F., Miyaoka Y., Miyajima A, & Tanaka M. (2014). Oncostatin M Maintains the Hematopoietic Microenvironment in the Bone Marrow by Modulating Adipogenesis and Osteogenesis. PLoS ONE, 9(12), e116209.
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5

In vivo EPO Regulation by Compound VII

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Example 6

Erythropoietin (EPO) is one of the earliest described and most sensitive HIF target genes; being positively regulated at the transcription level. Here, the ability of compound VII to regulated EPO levels in vivo was examined. Sixteen-week-old C57BL/6 male mice were treated intraperitoneally (i.p.) with Betulinic Acid (60 mg/kg) or compound VII (30 mg/kg or 60 mg/kg). Blood samples were taken under general anaesthesia 4 hours after treatment and the circulating levels of EPO in plasma were quantified using a mouse EPO ELISA kit (R&D Systems) according to manufacturer's instructions. EPO values represent the mean±SEM (n=3).

As shown in FIG. 7 in vivo administration of compound VII, as a representative of the compounds described in the present invention, in mice (30 and 60 mg/kg/day) strongly increased circulating EPO plasma levels. In contrast Betulinic acid (BA), the parental of compound VII, did not influence the levels of EPO in plasma.

US10766855B2. Hydroxamate triterpenoid derivatives (2020-09-08). VIVACELL BIOTECHNOLOGY ESPANA S.L. [ES]. Inventors: Eduardo Munoz Blanco [ES], Alberto Minassi [IT], Maria Luz Bellido Cabello De Alba [ES], Giovanni Appendino [IT].
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6

Quantifying Molecular Markers in Mouse Models

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Kallistatin levels were measured using a human kallistatin ELISA kit (R&D Systems, Minneapolis, MN) and a mouse kallistatin ELISA kit (Fine Biological Technology, Wuhan, China); fibronectin was measured using a mouse fibronectin ELISA kit (Assaypro, St. Charles, MO), ICAM‐1 using a mouse ICAM‐1 ELISA kit (R&D systems, Minneapolis, MN), Ang II by a ELISA kit (Enzo Life Sciences, Farmingdale, NY), and erythropoietin (EPO) using a mouse EPO ELISA kit (R&D systems, Minneapolis, MN). Western blot analysis was performed as described.30 The primary antibodies used were purchased from the following companies: mouse kallistatin (SerpinA3C) (R&D Systems, Minneapolis, MN); TGF‐β1 (R&D Systems, Minneapolis, MN), TβR‐I and TβR‐II (Novus Biologicals, Littleton, CO); CTGF (Santa Cruz, Dallas, TX); TNF‐α (Cell Signaling Technology, Danvers, MA); 3‐NT, NOX4, and NOX2 (Abcam, Cambridge, MA); ACE (R&D systems, Minneapolis, MN); HIF‐1α (Novus Biologicals, Littleton, CO) and β‐actin (Sigma‐Aldrich, St. Louis, MO).
Yang Y., He X., Cheng R., Chen Q., Shan C., Chen L, & Ma J. (2020). Diabetes‐induced upregulation of kallistatin levels exacerbates diabetic nephropathy via RAS activation. The FASEB Journal, 34(6), 8428-8441.
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7

Measuring Biomarkers in Experimental Models

Cited 1 time
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Kallistatin levels were measured using a human kallistatin ELISA kit (R&D Systems, Minneapolis, MN) and a mouse kallistatin ELISA kit (Fine Biological Technology, Wuhan, China); fibronectin was measured using a mouse fibronectin ELISA kit (Assaypro, St. Charles, MO), ICAM-1 using a mouse ICAM-1 ELISA kit (R&D systems, Minneapolis, MN), Ang II by a ELISA kit (Enzo Life Sciences, Farmingdale, NY), and erythropoietin (EPO) using a mouse EPO ELISA kit (R&D systems, Minneapolis, MN). Western blot analysis was performed as described (30 (link)). The primary antibodies used were purchased from the following companies: mouse kallistatin (SerpinA3C) (R&D Systems, Minneapolis, MN); TGF-β1 (R&D Systems, Minneapolis, MN), TβR-I and TβR-II (Novus Biologicals, Littleton, CO); CTGF (Santa Cruz, Dallas, TX); TNF-α (Cell Signaling Technology, Danvers, MA); 3-NT, NOX4 and NOX2 (Abcam, Cambridge, MA); ACE (R&D systems, Minneapolis, MN); HIF-1α (Novus Biologicals, Littleton, CO) and β-actin (Sigma-Aldrich, St. Louis, MO).
Yang Y., He X., Cheng R., Chen Q., Shan C., Chen L, & Ma J. (2020). Diabetes‐induced upregulation of kallistatin levels exacerbates diabetic nephropathy via RAS activation. The FASEB Journal, 34(6), 8428-8441.
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8

Determination of Plasma Epo Levels

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Plasma Epo levels were determined using a mouse Epo ELISA kit (R&D systems) following manufacturer's instructions.
Neves J., Leitz D., Kraut S., Brandenberger C., Agrawal R., Weissmann N., Mühlfeld C., Mall M.A., Altamura S, & Muckenthaler M.U. (2017). Disruption of the Hepcidin/Ferroportin Regulatory System Causes Pulmonary Iron Overload and Restrictive Lung Disease. EBioMedicine, 20, 230-239.
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9

Hemoglobin, Hematocrit, and EPO Quantification

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Blood sampling was performed on rats to measure total hemoglobin (Avoximeter 4000; A-VOX Systems, San Antonio, TX) and hematocrit (HAEMATOKRIT 210, Hettich, Tuttlingen, Germany) at the end of the protocol. Because of the kit compatibility, C57BL/6J mice received ip injection of DMOG or EDHB (100 and 200 mg/kg body wt) and were killed 8 h after administration for plasma EPO determination using a mouse EPO ELISA kit (R&D Systems, Minneapolis, MN). Previous study on EPO induction with PHI showed that EPO level was close to the maximal concentration 8 h after injection (15) .
Favier F.B., Britto F.A., Ponçon B., Begue G., Chabi B., Reboul C., Meyer G, & Py G. (2016). Endurance training prevents negative effects of the hypoxia mimetic dimethyloxalylglycine on cardiac and skeletal muscle function. Journal of applied physiology (Bethesda, Md. : 1985), 120(4).
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