Bs 0368g af555

The immunocytochemistry of C2C12 cells was performed as previously described [19 (link)]. The primary antibody used was MyHC (MAB4470, R&D Systems, Minneapolis, MN, USA). The secondary antibody used was goat anti-mouse IgM/Alexa Fluor 555 antibody (bs-0368G-AF555, Bioss, Beijing, China). Images were captured with a microscope at 20 X magnification (6 fields per sample captured), and the myotube diameter and myotube fusion index were counted by using IPP.

For staining of C2C12 cell, C2C12 cell was punched in 0.4% Triton for 10 min and then blocked for 1 h with a slowly shaking at room temperature. The sections were then incubated with primary antibody at room temperature overnight in a wet box. Goat anti-rabbit FITC (bs-0295G, Bioss), goat anti-mouse IgM/Alexa Fluor 555 antibody (bs-0368G-AF555, Bioss), goat anti-rabbit Flour 555 (bs-0295G, Bioss), goat anti-mouse FITC (bs-50950, Biowarld), rabbit anti-goat IgG FITC (bs-0294R, Bioss), and corresponding second antibodies were supplied for use. A Nikon Eclipse Ti-s microscope was used to take photos of these sections. Images of fluorescent intensity were analyzed with Nis-Elements BR software (Nikon Instruments, Tokyo, Japan).