Dulbecco s phosphate buffer 7.4 ph

Quercetin (Qu) was procured from Sisco Research Laboratories Pvt. Ltd., India. Soluplus and poloxamer 407 were obtained as a gift sample from the BSFA (British Scientific Fiction Association Lit.). E-TPGS was gifted from Shilpa medicare Ltd., Bangalore, India. Dulbecco’s Modified Eagle’s medium (DMEM), Trypsin EDTA, Dulbecco’s phosphate buffer 7.4 pH, Penicillin–Streptomycin, Fetal bovine serum (FBS), and glutamate were purchased from Thermo Fisher Scientific (Pittsburgh, PA, USA). Cell culture plasticware was obtained from Techno Plastic Products AG (TPP, Trasadingen, Switzerland). Dimethyl Sulfoxide (DMSO) molecular grade and 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide) (MTT) were purchased from Sigma-Aldrich, USA. The water used in the experiment was RO purified, and all other solvents and chemicals used were of analytical grade.

Soluplus and Resveratrol were obtained as a plentiful gift from BASF, Tifton, GA, USA. Paclitaxel was gifted by Shilpa Medicare Ltd., Bangalore, India. Dulbecco’s Modified Eagle’s medium (DMEM), Trypsin EDTA and Dulbecco’s phosphate buffer 7.4 pH, Penicillin-Streptomycin and glutaMAX^TM were purchased from Thermo Fisher Scientific (Pittsburgh, PA, USA). Di-methyl Sulfoxide (DMSO) molecular grade and 3-(4, 5-Dimethylthiazol-2-yl)-2, 5- diphenyl tetrazolium bromide) (MTT) were purchased from Sigma-Aldrich, St. Louis, MO, USA.
Animal glioma cell line (C6 ATCC^® CCL-107) was obtained from American Type Culture Collection (ATCC, Rockville, MD, USA). Cells were maintained in complete DMEM media, supplemented with 10% v/v FBS and 1% v/v Penicillin-Streptomycin and glutaMAXTM solution at 37 ± 0.5 °C with 5% carbon dioxide (CO2) in an incubator [14 (link)].