Anti rad50

Whole cell and nuclear extracts were prepared as described previously^{63 (link)}. Western blot analysis was performed as described^{64 (link)} with the following primary antibodies: anti-TRF1 (#5745, 0.5 μg/ml)^{63 (link)}, anti-TRF2 (4A794, 2.5 μg/ml, Novus Biologicals, Littleton, CO, USA), anti-POT1 (#978, 1:1,000, provided by Dr. Titia de Lange), anti-TIN2 (1:1,500, provided by Dr. Zhou Songyang), anti-TPP1 (#467, 1:1,000, provided by Zhou Songyang), anti-RAP1 (1:1,000, provided by Zhou Songyang), anti-RIF1 (#1060, 1:1,000, provided by Titia de Lange), anti-dyskerin (H-300, 2 μg/ml, Santa Cruz Biotechnology, Dallas, TX, USA), anti-MRE11 (12D7, 1:500, GeneTex, Irvine, CA, USA), anti-RAD50 (13B3, 1:1,000, GeneTex), anti-NBS1 (#3002, 1:1,000, Cell Signaling Technology, Danvers, MA, USA), anti-BLM (ab476, 1:2,000, Abcam, Cambridge, UK), anti-WRN (ab200, 1:2,000, Abcam), anti-ATM (2C1, 2 μg/ml, Santa Cruz Biotechnology), anti-PARP-1 (C2-10, 1:2,000, Pharmingen, Franklin Lakes, NJ, USA), anti-tankyrase-1 (H-350, 2 μg/ml, Santa Cruz Biotechnology), anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH; RDI-TRK5G4-6C5, 0.5 μg/ml, Research Diagnostics, Flanders, NJ, USA), anti-α-tubulin (B-5-1-2, 1:1,000, Sigma), anti-lamin A/C (636, 2 μg/ml, Santa Cruz Biotechnology) and anti-hTERT (1531-1/Y182, 1:1,000, Abcam). Images were processed with Photoshop CS5 (Adobe).