A 1 μl aliquot of carboxylate‐modified polystyrene, fluorescent yellow‐green latex beads with a mean particle size of 1 μm (Sigma‐Aldrich, L4655) was diluted into 1ml of phosphate buffer. A 5 μl of this stock solution was added to the protein solution and gently mixed to disperse the particles. A 80 μl of the bead and protein solution was placed on a cavity slide (Brand GmBH, 0.6–0.8mm depth) and sealed with a coverslip using nail varnish. Movies of the motion of the particles were taken using a Nikon Eclipe Ti microscope equipped with a Hamamatsu Orca‐Flash 4.0 CCD camera. Images were acquired using μ‐manager software at a framerate of 10 fps (Edelstein et al., 2010 ). Movies were then analysed using TrackPy (available from github.com/soft‐matter/trackpy).
Cavity slide
Manufactured by Brandel Inc
Sourced in Germany
The Cavity Slide is a laboratory equipment used for microscopic examination of small samples. It provides a secure and contained environment for the specimen during analysis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using cavity slide
1
Tracking Fluorescent Beads Motion
2
Confocal Microscopy of Milk Lipids and Proteins
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Staining procedures and CLSM analysis were based on the method of Ong et al. (2010) . Briefly, 10 mL of each milk was added to a solution of 4 mg fast green (Sigma-Aldrich) in 150 μL acetone (Sigma-Aldrich) to stain fat globules. After holding for 30 min, a solution of 2 mg of Nile red (TCI America, Portland, OR) in 150 μL of acetone was added to the sample and held for another 30 min to stain milk proteins. Then, 100 μL of the stained sample was transferred to a cavity slide (BRAND, Wertheim, Germany) and covered with a glass coverslip with 2 drops of oil in the center of the coverslip. Samples were viewed at 22 ± 2°C using a confocal laser scanning microscope (Olympus Corporation, Tokyo, Japan) equipped with an oil immersion 60× lens. Nile red was excited at a wavelength of 488 nm and fast green FCF at 633 nm.
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