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4 protocols using sodium nitrate

1

Chemical Standards for Nitrite and Nitrate Analysis

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Nitrite ion standard solution (NaNO2) and nitrate ion standard solution (NaNO3) were acquired from Sigma–Aldrich Co. (USA). Sodium nitrite, sodium nitrate, and tert-butanol were purchased from Samchun Pure Chemical Co. ltd. (KOR). Potassium biphthalate (C8H5KO4) was acquired from Daejung Chemical &Metals Co. ltd. (KOR). Potassium iodide (KI) and ammonium molybdate [(NH4)2MoO4] were purchased from Junsei Chemical Co. ltd. (JPN). All chemicals were used as received.
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2

Polymer-based Functionalized Graphite

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Poly(ethylene glycol) (Mn 2000, 6000, 12,000 g mol−1) (PEG2K, PEG6K and PEG12K), triethylamine (TEA), dichloromethane (DCM), graphite (flake), sodium citrate, N-isopropyl-amine (NIPAm), ammonium persulfate (APS), N,N,N′,N′-tetramethylethylenediamine (TEMED), sodium citrate and bovine serum albumin (BSA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Potassium persulfate, phosphorus pentoxide, sulfuric acid (97%), sodium nitrate, potassium permanganate, hydrogen peroxide (30%), and diethyl ether were purchased from Samchun Chemicals Co. (Seoul, Korea).
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3

Electrochemical Synthesis of Graphene Oxide

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D-glucose (97.5–102.0%), sulfuric acid (95.0–97.0%), hydrochloric acid fuming (37.0%), and hydrogen peroxide (30.0%) were purchased from Merck meanwhile urea (98.0%), potassium permanganate (99.3%), sodium nitrate (99.0%), and borax 99.0–103.0%) were supplied from Samchun Chemicals. Moreover, PVA and graphite nanoparticles were obtained from Japan Vam & Poval Co. (POVAL™ JP-24) and US Research Nanomaterials, Inc. (C, 400 nm–1.2 µm, 99.9%), respectively.
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4

Synthetic Media Composition for Microbial Growth

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Sucrose, potassium chloride, sodium nitrate, magnesium sulfate heptahydrate, and potassium phosphate were obtained from Samchun Pure Chemicals Co. Ltd. (Pyeongtaek, Gyeonggi-do, Korea). L-Threitol was purchased from Tokyo Chemical Industry Co. Ltd. (Tokyo, Japan). Peptone, yeast extract and tryptone soya broth (TSB) were purchased from Becton Dickinson (Sparks, MD, USA). Acetonitrile and methanol were purchased from J.T.Baker (Phillipsburg, NJ, USA). All the other chemicals were obtained from Sigma-Aldrich (St. Louis, MO, USA).
The synthetic medium contained 1% leucine, 2%, Sucrose, 0.5% Peptone, 0.1% yeast extract, 0.1% potassium phosphate, 0.05% of potassium chloride and magnesium sulfate heptahydrate, trace element (5 g of citric acid, 1 g of ZnSO4·H2O, 5 g of Fe(NH4)2 (SO4)2·6H2O, 250 mg of CuSO4·5H2O, 50 mg of boric acid, 50 mg of MnSO4 and 50 mg of Na2MoO4·2H2O in distilled water), and vitamin solution (4 g of inositol, 200 mg of pantothenic acid, 200 mg of choline, 100 mg of thiamine, 75 mg of pyridoxine, 75 mg of nicotinamide, 30 mg of riboflavin, 5 mg of p-aminobenzoic acid, 5 mg of ascorbic acid, 5 mg of folic acid and 5 mg of biotin in 50% ethanol).
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