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Mega preparation kit

Manufactured by Qiagen
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The Mega preparation kit is a laboratory equipment product designed for the large-scale isolation and purification of plasmid DNA. It provides a reliable and efficient method for obtaining high-quality plasmid DNA samples suitable for various downstream applications.

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Lab products found in correlation

Il 12 and il 2 recombinant proteins, by R&D Systems (2 mentions) Ph 797804, by Selleck Chemicals (2 mentions) Carboxyfluorescein succinimidyl ester (cfse), by Thermo Fisher Scientific (2 mentions) Pd98059, by Selleck Chemicals (2 mentions) Pki 587, by Selleck Chemicals (2 mentions) Anacardic acid, by Merck Group (1 mentions) Cyclophosphamide, by Merck Group (1 mentions) Methotrexate, by Merck Group (1 mentions) Trichostatin a, by Merck Group (1 mentions) Sodium butyrate, by Merck Group (1 mentions) Pegfp n1 vector, by Takara Bio (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions) Chloroquine, by Merck Group (1 mentions) Ifosfamide, by Merck Group (1 mentions)

Spelling variants of this product

Qiagen Mega Kit (6 citations)

4 protocols using mega preparation kit

1

Dox and IL-12 Gene Therapy Protocol

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The mice were treated with a combination regimen of Dox plus an IL-12 gene construct. The IL-12 gene construct was obtained from Valentis, Inc. (Vilnius, Lithuania); the backbone of this construct was described in a previous publication [44 (link)]. The control plasmid DNA consisted of the same construct with the IL-12 gene deleted. Plasmid DNA was prepared by using the endotoxin-free Mega preparation kit from Qiagen, Inc. (Valencia, CA) according to the manufacturer’s instructions. Doxorubicin (Bedford Laboratories, Bedford, OH) was purchased from the pharmacy at the Louisiana State University School of Veterinary Medicine.
Hu J., Zhu S., Xia X., Zhang L., Kleinerman E.S, & Li S. (2014). CD8+T cell–specific induction of NKG2D receptor by doxorubicin plus interleukin-12 and its contribution to CD8+T cell accumulation in tumors. Molecular Cancer, 13, 34.
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2

Molecular Constructs and Therapeutic Agents in Cancer Research

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The IL12 DNA construct was purchased from Valentis, Inc. (Vilnius, Lithuania). Rae-1 constructs were PCR-amplified from mouse cDNA and cloned into pEGFP N1 vector (Clontech, Mountain View, CA). Mouse IL15 and IL4 mRNAs were purchased from Open Biosystems (Thermo Scientific, Two Rivers, WI). mIL21 pORF9 mRNA was purchased from Invivogen (San Diego, CA). mIL18 was purchased from OriGene Technologies (Rockville, MD). mIFNγ was PCR-amplified from mouse genomic DNA and mouse spleen cDNA, respectively. All constructs were confirmed by sequence analyses. DNA was prepared by using the endotoxin-free Mega preparation kit from Qiagen, Inc. (Valencia, CA) by following the manufacturer’s instructions.
Doxorubicin (Bedford Laboratories, Bedford, OH) and bleomycin (APP Pharmaceuticals, Schaumburg, IL) were purchased from the pharmacy at the Louisiana State University or The University of Texas MD Anderson Cancer Center. Cisplatin was purchased from Bristol Laboratories (Princeton, NJ). Cycloheximide, cyclophosphamide, chloroquine, methotrexate, and ifosfamide were purchased from Sigma-Aldrich (St. Louis, MO). Trichostatin A, sodium butyrate and anacardic acid were purchased from Sigma-Aldrich (St. Louis, MO). IL12 and IFNγ recombinant proteins were purchased from R&D systems (Minneapolis, MN).
Hu J., Bernatchez C., Zhang L., Xia X., Kleinerman E.S., Hung M.C., Hwu P, & Li S. (2017). Induction of NKG2D Ligands on Solid Tumors Requires Tumor-Specific CD8+ T Cells and Histone Acetyltransferases. Cancer immunology research, 5(4), 300-311.
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3

Preparing IL-12 DNA Construct and Reagents

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The IL-12 DNA construct was purchased from Valentis, Inc. (Vilnius, Lithuania) and was confirmed in-house by sequence analyses. DNA was prepared by using an endotoxin-free Mega preparation kit from Qiagen, Inc. (Valencia, CA) according to the manufacturer’s instructions. Doxorubicin (Bedford Laboratories, Bedford, OH) was purchased from the pharmacy at MD Anderson Cancer Center. NF-κB pathway inhibitors S3I-201, PH797804, PD98059, PKI-587, and QNZ were purchased from Selleckchem (Houston, TX). IL-12 and IL-2 recombinant proteins were purchased from R&D Systems (Minneapolis, MN). Carboxyfluorescein succinimidyl ester (CFSE) was purchased from Life Technologies (Grand Island, NY).
Hu J., Xia X., Gorlick R, & Li S. (2019). Induction of NKG2D ligand expression on tumor cells by CD8+ T cell engagement-mediated activation of nuclear factor-kappa B and p300/CBP-associated factor. Oncogene, 38(49), 7433-7446.
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4

Preparing IL-12 DNA Construct and Reagents

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The IL-12 DNA construct was purchased from Valentis, Inc. (Vilnius, Lithuania) and was confirmed in-house by sequence analyses. DNA was prepared by using an endotoxin-free Mega preparation kit from Qiagen, Inc. (Valencia, CA) according to the manufacturer’s instructions. Doxorubicin (Bedford Laboratories, Bedford, OH) was purchased from the pharmacy at MD Anderson Cancer Center. NF-κB pathway inhibitors S3I-201, PH797804, PD98059, PKI-587, and QNZ were purchased from Selleckchem (Houston, TX). IL-12 and IL-2 recombinant proteins were purchased from R&D Systems (Minneapolis, MN). Carboxyfluorescein succinimidyl ester (CFSE) was purchased from Life Technologies (Grand Island, NY).
Hu J., Xia X., Gorlick R, & Li S. (2019). Induction of NKG2D ligand expression on tumor cells by CD8+ T cell engagement-mediated activation of nuclear factor-kappa B and p300/CBP-associated factor. Oncogene, 38(49), 7433-7446.
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