The TP53 gene, exon 5 to exon 9 including exon–intron junctions, were amplified by polymerase chain reaction (PCR) using “p53 primers” (Nippon Gene) and Ex Taq DNA polymerase with 3′ exonuclease activity (TaKaRa Bio Inc., Tokyo, Japan). The PCR products were purified and used as templates for cycle sequencing reactions with Big Dye Terminator Cycle Sequencing Kit Ver.1.0 (Applied Biosystems, Foster City, CA). Mutations found in a PCR product were verified by reverse sequencing and reconfirmed in two independently amplified PCR products.
P53 primers
Manufactured by Nippon Gene
Sourced in Japan
P53 primers are oligonucleotide sequences designed to amplify the p53 gene. The p53 gene is a tumor suppressor gene that plays a crucial role in regulating cell growth and division. The P53 primers are used in molecular biology techniques, such as polymerase chain reaction (PCR), to detect and analyze the p53 gene.
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Lab products found in correlation
2 protocols using p53 primers
1
TP53 Gene Sequencing Protocol
2
Breast Cancer TP53 Gene Mutation Analysis
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We analyzed the TP53 gene mutation from 36 breast cancer specimens including 10 Wip1 protein-positive cases and 26 Wip1 protein-negative cases. The TP53 gene, exon 5 to exon 9 including exoneintron junctions, was amplified using PCR with p53 primers (Nippon Gene) and Ex Taq DNA polymerase with 3 0 exonuclease activity (TaKaRa Bio Inc, Tokyo, Japan). The p53 primers were as follows: exon 5 forward, 5 0 -TGCAGGAGGTGCTTACACATG-3 0 ; exon 5 reverse, 5 0 -TCCACTCGGATAAGATGCTG-3 0 ; exon 6 forward, 5 0 -GAAAATCTGGCACCACACCT-3 0 ; exon 6 reverse, 5 0 -GGAGGGCCACTGACAACCA-3 0 ; exon 7 forward, 5 0 -TGCCACAGGTCTCCCCAAGG-3 0 ; exon 7 reverse, 5 0 -GCA-CAGCAGGCCAGTGTGCA-3 0 ; exon 8-9 forward, 5 0 -TTGGGAGTAGATGGAGCCT-3 0 ; and exon 8-9 reverse, 5 0 -AGTGTTAGACTGGAAACTTT-3 0 . The PCR products were purified and used as templates for cycle sequencing reactions with the Big Dye Terminator Cycle Sequencing Kit version 1.1 (Applied Biosystems, Foster City, CA). Mutations detected in a PCR product were verified using reverse sequencing and reconfirmed in 2 independently amplified PCR products. 39
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