Mice bearing 4T1 tumors were treated as described above, sacrificed (n=3 per time point) at 7, 14 and 28 days after treatment and serum was collected for anti-MV antibody studies. The MV-specific IgG titer was measured by an indirect immunofluorescence test using MV antigen substrate slides (Bion Enterprises, Des Plaines, IL) according to the manufacturer's instructions, and performed as described (43 (link), 44 (link)). The protocol was modified using goat anti-mouse IgG fluorescein isothiocyanate-conjugated antibodies (Invitrogen) for detection of murine antibodies.
Goat anti mouse igg fluorescein isothiocyanate conjugated antibodies
Manufactured by Thermo Fisher Scientific
Goat anti-mouse IgG fluorescein isothiocyanate-conjugated antibodies are secondary antibodies that specifically bind to mouse immunoglobulin G (IgG) molecules. The antibodies are labeled with the fluorescent dye fluorescein isothiocyanate (FITC), which allows for the detection and visualization of target proteins in various immunoassays and imaging applications.
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2 protocols using goat anti mouse igg fluorescein isothiocyanate conjugated antibodies
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Mice bearing 4T1 tumors were treated as described above, sacrificed (n=3 per time point) at 7, 14 and 28 days after treatment and serum was collected for anti-MV antibody studies. The MV-specific IgG titer was measured by an indirect immunofluorescence test using MV antigen substrate slides (Bion Enterprises, Des Plaines, IL) according to the manufacturer's instructions, and performed as described (43 (link), 44 (link)). The protocol was modified using goat anti-mouse IgG fluorescein isothiocyanate-conjugated antibodies (Invitrogen) for detection of murine antibodies.
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