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Rosa26nicd

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The Rosa26NICD is a genetically engineered mouse line designed for targeted gene expression studies. It is a Cre-inducible Notch Intracellular Domain (NICD) transgenic model that allows for the conditional activation of the Notch signaling pathway in specific cell types or tissues.

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Lab products found in correlation

Rosa26 eyfp, by Jackson ImmunoResearch (2 mentions) Lck cre 003802, by Jackson ImmunoResearch (2 mentions) Cd8a cre 008766, by Jackson ImmunoResearch (2 mentions) Polyinosinic polycytidylic acid pipc, by Merck Group (2 mentions)

2 protocols using rosa26nicd

1

Conditional Knockout of Dnmt3a and Dnmt3b in Mice

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Animal procedures were approved by the Institutional Animal Care and Use Committee (IACUC) and conducted in accordance with Washington University School of Medicine institutional guidelines. Mice were C57Bl/6 background, Mx1-Cre:Dnmt3afl/fl and Mx1-Cre:Dnmt3afl/fl;Dnmt3bfl/fl mice have been described (16 ). Recombination of floxed alleles was mediated by six intraperitoneal injections (300μg/mouse) of polyinosinic-polycytidylic acid (pIpC; Sigma, St. Louis, MO, USA) in PBS every other day. The following strains were obtained from the Jackson Laboratory (Bar Harbor, ME, USA) - Lck-CRE (003802), CD8a-CRE (008766), Rosa26NICD (008159), Rosa26EYFP (006148) and Nr4a1KO (006187).
Kramer A.C., Kothari A., Wilson W.C., Celik H., Nikitas J., Mallaney C., Ostrander E.L., Eultgen E., Martens A., Valentine M.C., Young A.L., Druley T.E., Figueroa M.E., Zhang B, & Challen G.A. (2017). Dnmt3a Regulates T-cell Development and Suppresses T-ALL Transformation. Leukemia, 31(11), 2479-2490.
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2

Conditional Knockout of Dnmt3a and Dnmt3b in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Animal procedures were approved by the Institutional Animal Care and Use Committee (IACUC) and conducted in accordance with Washington University School of Medicine institutional guidelines. Mice were C57Bl/6 background, Mx1-Cre:Dnmt3afl/fl and Mx1-Cre:Dnmt3afl/fl;Dnmt3bfl/fl mice have been described (16 ). Recombination of floxed alleles was mediated by six intraperitoneal injections (300μg/mouse) of polyinosinic-polycytidylic acid (pIpC; Sigma, St. Louis, MO, USA) in PBS every other day. The following strains were obtained from the Jackson Laboratory (Bar Harbor, ME, USA) - Lck-CRE (003802), CD8a-CRE (008766), Rosa26NICD (008159), Rosa26EYFP (006148) and Nr4a1KO (006187).
Kramer A.C., Kothari A., Wilson W.C., Celik H., Nikitas J., Mallaney C., Ostrander E.L., Eultgen E., Martens A., Valentine M.C., Young A.L., Druley T.E., Figueroa M.E., Zhang B, & Challen G.A. (2017). Dnmt3a Regulates T-cell Development and Suppresses T-ALL Transformation. Leukemia, 31(11), 2479-2490.
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