Mbs701668

According to the manufacturer’s instructions, plasma immunoglobulin (Ig) A, G, and M levels were measured using commercial ELISA kits (Shanghai Jianglai Biotechnology Co., Ltd., Shanghai, China). The assays limits of detection, limits of quantification, intra-assay CV%, and interassay CV% are presented in Table 3 according to the manufacturer-provided data.
Plasma corticosterone level was quantified using chicken-specific quantitative competitive ELISA kit (MBS701668; MyBioSource, San Diego, CA, USA). According to the manufacturer, the CV% for the intra- and interassay were <8% and <10%, respectively, with a detection range of 0.5–20 ng/mL. Meanwhile, plasma total protein (TP), triglyceride (TG), and total cholesterol levels were quantified according to the kits’ protocols using quantitative colorimetric assays (ab102535, ab65336, and ab65390, respectively; Abcam, Waltham, MA, USA). As for liver enzyme activity, alanine amino transferase (ALT) and aspartate amino transferase (AST) activities were determined using commercial kits applying enzyme activity colorimetric assays (ab241035 and ab105135, respectively; Abcam, Waltham, MA, USA).

Plasma corticosterone concentrations were determined (n = 10; 2 samples per replicate in each group) using a specific chicken corticosterone ELISA kit (MBS701668; MyBioSource, San Diego, CA, USA). The intra- and inter-assay coefficient of variations (CV) were <8% and 10%, respectively. The assay sensitivity was less than 0.0625 ng/mL, with a dynamic range of 0.5 to 20 ng/mL. The measurements were recorded by an automated microplate reader (Model 550 Microplate Reader, Bio-Rad Laboratories Inc., Hercules, CA, USA).