Eight-week-old male C3H/HeNCrl mice were obtained from Beijing Vital River Laboratory Animal Technology Company. Amoebic colitis was induced by directly inoculating 1 × 106 axenic E. histolytica SAW755CR trophozoites into the cecum (n = 6), and the control group received the same volume of medium without trophozoites (n = 4). Six days after inoculation, the cecum tissues were harvested and fixed in 4% paraformaldehyde, followed by paraffin embedding. Tissue sections were stained with hematoxylin and eosin or periodic acid-Schiff (PAS) stain for histopathological analysis. Furthermore, immunohistochemical staining was performed using anti-PKM2 monoclonal antibody (Cell Signaling Technology, D78A4; Boston, MA, USA) to detect the expression level of PKM2 in the cecum.
All animal experiments were performed in strict accordance with the guidelines of the Regulations for the Administration of Affairs Concerning Experimental Animals (1988.11.1) and approved by the Institutional Animal Care and Use Committee (permit no. 20160225-097). All efforts were made to minimize animal suffering.
All animal experiments were performed in strict accordance with the guidelines of the Regulations for the Administration of Affairs Concerning Experimental Animals (1988.11.1) and approved by the Institutional Animal Care and Use Committee (permit no. 20160225-097). All efforts were made to minimize animal suffering.