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5 protocols using mf 319 developer

1

Fabrication of PDMS Microcontact Printing Stamps

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FN nanofibers were prepared by adapting the SIA approach described previously.20 , 21 (link) Briefly, poly(dimethylsiloxane) (PDMS) stamps for microcontact printing, patterned with either 1 cm long, 50 µm wide raised lines or 1 cm long, 10 µm wide raised lines were fabricated by first spincoating glass wafers with SPR 220.3 positive photoresist (Microchem). The photoresist was then exposed to UV light through a photomask and developed using MF-319 developer (Microchem). A negative of the patterned photoresist wafer was formed by casting PDMS prepolymer (Sylgard 184, Dow Corning) over it and curing it at 65° C for 4 hours. The cured PDMS was then peeled off the glass wafer and 1 cm2 stamps for microcontact printing were cut out.
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2

Polystyrene Bead Synthesis and Characterization

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Carboxylate-coated
polystyrene
bead solutions (2.6% w/v, 1 μm diameter) were purchased from
Polysciences (Warrington, PA). Shipley S1808 photoresist, Thinner
P, and MF-319 developer were purchased from Microchem (Newton, MA).
Clean Earth Chemicals 24K gold plating solution (Grobet USA, Carlstadt,
NJ) was used as received. Fisher Premium glass microscope slides (1
mm thick) were used as substrates. Magnetite nanoparticles of 20 nm
(20 mg/mL in citrate buffer) were purchased from nanoComposix (San
Diego, CA) and diluted 1000-fold in water prior to use.
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3

Fabrication of PDMS Microcontact Stamps

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Polydimethylsiloxane (PDMS) stamps for microcontact printing consisting of an array of 20 μm wide, 200 μm long rectangles were fabricated according to published methods.21 -23 (link) Briefly, glass wafers were spincoated with SPR 220.3 positive photoresist (Microchem), exposed to UV light through a transparency-based photomask and developed using MF-319 developer (Microchem). Sylgard 2184 PDMS (Dow Corning) was then mixed in a 10:1 base to curing agent ratio, mixed and degassed and then poured on the patterned photoresist and cured at 65°C for 4 hours. PDMS were then cut out and sonicated in a 50% ethanol solution for 45 minutes and dried under a stream of nitrogen.
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4

Synthesis and Functionalization of Iron Oxide Nanoparticles

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Iron (III) chloride hexahydrate was purchased from Across (99%) and sodium oleate from TCI America (97%). Neutravidin protein, Alexa Fluor 647-NHS ester and Biotin-NHS ester were purchased from Thermo Fisher Scientific. LOR photoresist and MF-319 developer were obtained from Microchem and S1805 photoresist was obtained from Microposit. Other reagents and chemicals were purchased from Sigma-Aldrich and used as received unless otherwise stated.
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5

Fabrication of Microfluidic Devices

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Chemicals and materials were obtained from the following sources and used without further purification: 2'-Deoxyadenosine-5'-monophosphate (Alfa Aesar); Thymidine-5'-monophosphate disodium salt (Alfa Aesar); 2'-Deoxycytidine-5'-monophosphate (Alfa Aesar); 2'-Deoxyguanosine-5'-monophosphate disodium salt hydrate (Alfa Aesar); potassium chloride (1 and 3 m, Sigma-Aldrich); Tris-EDTA (100×, Sigma-Aldrich); Tris-Borate-EDTA (10×, Sigma-Aldrich); silver (Ag) wires (>99%, Sigma-Aldrich); S1813 photoresist (MicroChem); MF319 developer (MicroChem); potassium hydroxide (KOH) pellets (Fisher Scientific); hydrofluoric acid (HF) (Sigma-Aldrich); Fluorolink MD 700 (Solvay); 2-hydroxy-2-methylpropiophenone (Sigma-Aldrich); NOA72 (Norland Products); poly(ethylene glycol) diacrylate (PEGDA) (Mn = 250, Sigma-Aldrich); 2,2-dimethoxy-2-phenylacetophenone (Sigma-Aldrich); Si wafers (P/B, resistivity 5-10 Ωcm, orientation of (100), and 525 ± 25 μm thickness) (WaferPro); polyethylene terephthalate (PET) with 250 μm thickness (Goodfellow); polymethylmethacrylate (PMMA) with a thickness of 250 μm (Goodfellow); polymethylmethacrylate (PMMA) of 1 mm thickness (ePlastics).
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