Igg alexa fluor 488 or 568 conjugated goat anti mouse or anti rabbit antibodies

The following primary antibodies were used: hybridoma mouse anti-α-catenin (5B11; undiluted), polyclonal rabbit anti-phospho-serine 641 αCat (21330; 1:300, Signalway Antibody), monoclonal mouse anti-GAPDH (9484; 1:5000, Abcam). Secondary antibodies for Western blotting included fluorescently labeled donkey anti-mouse and anti-rabbit antibodies (680RD or 800RD; 1:5000, LiCor Biosciences). Secondary antibodies for immunofluorescence included IgG Alexa Fluor 488 or 568-conjugated goat anti-mouse or anti-rabbit antibodies (1:200, Invitrogen). For R2/7 cells expressing αE-catenin-FLAG, the following antibodies were used: anti-DDDDK tag rabbit polyclonal antibody, which recognizes the FLAG tag (1:200, MBL), anti-ZO-1 mouse monoclonal antibody (T8-754; undiluted, a gift from Sa. Tsukita, Osaka University, Japan), anti-E-cadherin mouse monoclonal antibody (clone36/E-cadherin; 1:100, BD) and anti-E-cadherin rat monoclonal antibody (ECCD2; 1:200, a gift from M. Takeichi, Riken, Japan). Alexa Fluor 488-conjugated or 555-conjugated secondary antibodies (1:200) were purchased from Invitrogen. Alexa Fluor 647-conjugated phalloidin (1:50, Invitrogen) was used for staining actin filaments.