Catechin, rutin, pinocembrin, gallic acid, 2,2 difenil-1-picrylhydrazyl (DPPH), Folin-Ciocalteu phenol reagent, Dimetilsulfoxide (DMSO), 3,5-dinitrobenzoic acid, sodium nitrite, aluminum chloride, ascorbic acid, 2,4,6-tripyridyl-s-triazine, riboflavin, nitroblue tetrazolium and phosphate saline buffer were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Sodium and pottasium hydroxide reactive grade, acetic anhydride, sodium carbonate, sodium acetate were obtained from J. T. Baker (Deventer, Holland). Absolute methanol and ethanol, acetic acid, sudan III reactive, glycerin, sodium chloride, ferric chloride, ninhydrin, chloroform, sulfuric acid, magnesium metal, amyl alcohol, iron chloride, hexane and ethylenediaminetetraacetic acid (EDTA) from Fagalab (Edo. de Mexico, Mexico), hydrochloric acid, picric acid from Fermont (Monterrey, Mexico), antimony chloride (Carr Price reactive) and Dragendorff reagent from Fluka (St. Gallen, Switzerland), fehling A and fehling B reactives from Meyer Reagents (Tlahuac, Mexico) and distilled water with mili-Q system (Millipore, Bedford, MA, USA) was used.
Dimetilsulfoxide
Manufactured by Merck Group
Sourced in United States
Dimethyl sulfoxide (DMSO) is a colorless, odorless, and polar aprotic solvent commonly used in various laboratory applications. It has a high boiling point and is known for its ability to dissolve a wide range of organic and inorganic compounds. DMSO is often utilized as a solvent and carrier in various chemical, biological, and pharmaceutical research studies.
Automatically generated - may contain errors
Lab products found in correlation
Folin ciocalteu, by Merck Group (2 mentions)
Nitroblue tetrazolium, by Merck Group (1 mentions)
Phosphate saline buffer, by Merck Group (1 mentions)
Catechin, by Merck Group (1 mentions)
Folin ciocalteu phenol reagent, by Merck Group (1 mentions)
Rutin, by Merck Group (1 mentions)
Riboflavin, by Merck Group (1 mentions)
Aluminum chloride, by Merck Group (1 mentions)
Sodium nitrite, by Merck Group (1 mentions)
Mili q system, by Merck Group (1 mentions)
2 4 6 tripyridyl s triazine, by Merck Group (1 mentions)
Pinocembrin, by Merck Group (1 mentions)
Ascorbic acid, by Merck Group (1 mentions)
Gallic acid, by Merck Group (1 mentions)
3 5 dinitrobenzoic acid, by Merck Group (1 mentions)
2 2 difenil 1 picrylhydrazyl, by Merck Group (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide, by Merck Group (1 mentions)
Rpmi 1640, by Merck Group (1 mentions)
Sodium bicarbonate, by Merck Group (1 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (1 mentions)
5 protocols using dimetilsulfoxide
1
Phytochemical Screening and Antioxidant Assays
2
Antioxidant and Cytotoxic Evaluation
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BHA (butylated hydroxyanisole) and DPPH• (2,2-diphenyl-2-picrylhydrazyl radical) were obtained from Sigma Chemical Co (St Louis, MO, USA). Porcine trypsin was purchased from Roche (Barcelona, Spain). Tripan blue, dimetilsulfoxide (DMSO), “Minimum Essential Medium Eagle (MEM)” and RPMI-1640 culture media, mix of antibiotics and antimycotic, sodium piruvate, sodium bicarbonate, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), dichlorofluorescein diacetate, cisplatin, and potassium dichromate were purchased from Sigma-Aldrich (Madrid, Spain) and fetal bovine serum (FBS) was obtained from T.D.I. (Madrid, Spain). The phenolic standards eriodictyol-7-O-glucoside, naringenin-7-O-glucoside, luteolin-7-O-glucoside, quercetin-7-O-rutinoside, rosmarinic acid, and verbascoside were obtained from Extrasynthese (Genay Cedex, France). Ascorbic acid, formic acid and ethanol were purchased from Panreac (Barcelona). n-Hexane, methanol and acetonitrile, all with HPLC purity, were purchased from Lab-Scan (Lisbon, Portugal).
3
C9orf72 Repeat Expansion Analysis
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Genomic DNA was isolated from peripheral blood according to standard protocols. The repeat number of the GGGGCC hexanucleotides was determined using genotyping primers. Repeat-primed polymerase chain reaction (PCR) was performed in order to provide a qualitative assessment of the presence of C9orf72 repeat expansions, as previously described (Dejesus-Hernandez et al., 2011 (link)). Briefly, 200 ng of genomic DNA were used as template in a final volume of 28 μl containing 12.5 μl of FastStart PCR Master Mix (Roche Applied Science, USA), and a final concentration of 0.25 mM 7-deaza-dGTP (Roche Applied Science), 5% dimetil sulfoxide (Sigma-Aldrich, USA), 1M betaine (Sigma-Aldrich) and 1 μM of each primer. Sample analyses were performed on an ABI 3500 genetic analyzer (Applied Biosystems), and data evaluated using the GeneMapper software. Repeat expansions are known to produce a characteristic sawtooth pattern with a 6-bp periodicity, as previously described (Dejesus-Hernandez et al., 2011 (link)).
4
Antioxidant Capacity Evaluation Protocol
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Dimetilsulfoxide, quercetin, 5,5′-Dithiobis(2-nitrobenzoic acid), Acetylcholine iodide, 1-chloro-2,4-dinitrobenzeno, 2′,7′-Dichlorofluorescein diacetate (DCHF-DA), Folin-Ciocalteu, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid diammonium salt, sodium acetate, HEPES, minimum 99,5 % titration, 2,4,6-Tris(2-pyridyl)-5-triazine (TPTZ), methyl viologen dichloride hydrate (Paraquat), 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT), resazurin sodium salt, and albumin from bovine serum were purchased from Sigma-Aldrich (São Paulo, SP, Brasil). Potassium ferrocyanidetrihydrate, zinc acetate dihydrate, iron (II) ammonium sulfate hexahydrate, gallic acid monohydrate, aluminum chloride hexahydrate, potassium persulfate were purchased from Vetec Química Fina LTDA (Rio de Janeiro, RJ, BRA). All other reagents were of analytical grade.
5
Cellular Signaling Pathway Analysis
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Dimetil sulfoxide (DMSO), Folin-Ciocalteu, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid diammonium salt, sodium acetate, HEPES minimum 99.5% titration, albumin from bovine serum (BSA), reduced glutathione (GSH), oxidized glutathione, tetramethylethylenediamine (TEMED), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), D-Manitol, K2HPO4, KH2PO4, Triton X-100, β-mercaptoethanol, anti-rabbit immunoglobulin (HRP peroxidase-linked antibody), and carbonyl cyanide 4-(trifluoromethoxy) phenylhydrate (FCCP) were obtained from Sigma-Aldrich (São Paulo, SP, Brazil). SDS, acrylamide, bis-acrylamide, and hybond nitrocellulose were obtained from GE Healthcare Life Division (Uppsala, Sweden). Anti-phospho-p38 (Thr180/Tyr182) and total form, anti phospho-AKT (Thr308) and total form, anti-phospho PTEN, anti-phospho and total JNK1/2 (Thr183/Tyr185), anti-phospho ERK1/2 (Thr202/Tyr204) and anti-total-ERK1/2, and β-actin antibodies were purchased from Cell Signaling Technology (Danvers, MA). Poly (ADP)-ribose polymerase (PARP) antibody was obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Kit Caspase-Glo 3/7 was obtained from Promega (Madison, WI). All other reagents were commercial products of the highest purity grade available.
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