Modified Boyden chamber assays were conducted using 24-well Transwell polyester membrane filter inserts with 8 µm pores and 0.33 cm2 surface area (Corning Inc., Corning, NY, USA) at a density of 500,000 cells/ml per transwell (upper chamber). DMEM medium without FBS in the upper chamber and total DMEM medium with 10% FBS in the bottom chambers of the transwells. After culturing for 24 h, the cells from the upper chambers were removed, and the migrated cells on the undersides of the membranes were stained with crystal violet (Beyotime, Haimen, China). Migratory cells were imaged and counted in high power microscope micro-photographs (field area: 0.98 mm2) taken under bright light (Olympus Tokyo, Japan) using Image Pro Plus 6.0 software (Rockville, MD).
24 well transwell polyester membrane filter inserts
Manufactured by Corning
Sourced in United States
The 24-well Transwell polyester membrane filter inserts are a laboratory equipment product designed for cell culture applications. The inserts feature a polyester membrane that provides a permeable barrier, allowing for the study of cell migration and permeability. The product is available in a 24-well format, providing multiple experimental wells within a single unit.
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2 protocols using 24 well transwell polyester membrane filter inserts
1
Boyden Chamber Assay for Cell Migration
2
Transwell Migration Assay for Cell Motility
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Modified Boyden chamber assays were conducted using 24-well Transwell polyester membrane filter inserts with 8-μm pores and 0.33 cm2 surface area (Corning Inc., Corning, NY, USA) at a density of 500,000 cells/ml per transwell (upper chamber). The l -type calcium channel blocker verapamil (10 μM) and/or PF-573228 were used in the bottom chambers of the transwells. After culturing for 24 h, the cells from the upper chambers were removed, and the migrated cells on the undersides of the membranes were stained with crystal violet (Beyotime, Haimen, China). Migratory cells were imaged and counted in high power microscope micro-photographs (field area: 0.98 mm2) taken under bright light (Olympus Tokyo, Japan) using Image Pro Plus 6.0 software (Rockville, MD).
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