Angiogenesis array kit

CFSE far-red, CFSE green fluorescence dye, easYFlasks, and NUNC cell culture 24-well plates were purchased from Thermo Fisher Scientific (Loughborough, UK). Dextran (DEX) (MW 500 kDa) was purchased from Alfa Aesar (Heysham, Lancashire, UK), sodium chloride (99.8%) and calcium chloride were sourced from Eagle’s Modified Eagle Medium, and Trypsin-EDTA were sourced from Gibco (Loughborough, UK). Fetal bovine serum (FBS) was sourced from Labtech (Heathfield, UK) and Trypsin-EDTA was purchased from Lonza (UK). Endothelial cell culture medium was purchased from ATCC, HUVEC cell line was sourced from Promocell (Lutterworth, Leicestershire, UK), and Hep-G2 cell line was purchased from ECACC cell collection (Salisbury, UK). The MMP-2 ELISA kit was purchased from GE healthcare (Amersham, UK), the IGFBP, VEGF, IL-8, and HIF1-α DuoSet ELISA kits and angiogenesis array kit (ARY007) were all purchased from Bio-Techne (Abingdon, UK). The 2 wt% gelatin suspension was sourced from Sigma Aldrich (Gillingham, UK) and Matrigel was purchased from Corning (Flintshire, UK). Whey protein was sourced from No.1 Supplements (Suffolk, UK). Deionized water was purified by using MilliQ reverse osmosis water purification system (Merck, Millipore, Burlington, MA, USA). All the other chemicals were of analytical grade.