Agilent 6520 accurate mass q tof lc

Optical rotations were measured on a JASCO P-2000 automatic digital polarimeter. UV and CD spectra were recorded on a JASCO-815 CD spectrometer. IR spectra were measured on a Nicolet 5700 FT-IR microscope instrument (FT-IR microscope transmission). NMR spectra were obtained at 600 MHz for ¹H and 150 MHz for ¹³C, respectively, on a Bruker AVIIIHD 600 NMR spectrometer. ESIMS and HRESIMS data were measured using AB SCIEX QTOF MS (QSTAR Elite) and Agilent 6520 Accurate-Mass Q-TOF LC/MS spectrometers, respectively. Analytical HPLC was conducted on an Agilent 1260 infinity system equipped with a DAD-UV detector. Preparative HPLC was performed on a CXTH system (Beijing Chuangxintongheng instrument Co. Ltd., P.R. China), equipped with a UV3000 detector using a YMC-Pack ODS-A column (250 × 20 mm, 5 μm, Kyoto, Japan). Preparative TLC were performed on precoated TLC plates (400-500 μm thickness, HSGF254, Yantai Jiangyou Silica Gel Development Co. Ltd., Yantai, P.R. China). Polyamide (30–60 mesh, Changzhou Changfeng Chemical Factory, China), Silica gel (100–200, 200–300 mesh, Qingdao Marin Chemical Inc. Qingdao, P.R. China) and Sephadex LH-20 (GE), and ODS (50 μm, YMC, Japan) were used for column chromatography.