Collagen 1 tc flask

Immediately after the muscle biopsy, satellite cells were isolated and cultured into myoblasts as previously described ^{23 (link)}. Briefly, human skeletal muscle cells (HSkMCs) were thawed and grown in a humidified environment with 5% CO₂ at 37°C on collagen I TC flask (Greiner Bio-one, Monroe, NC) ^{23 (link)}. Myoblasts were sub-cultured onto a 12-well type I collagen-coated plate, 6-well type I collagen-coated plate (Corning, Corning, NY), 35 mm poly-d-lysine coated glass bottom dish (MatTek, Ashland, MA) or a seahorse XFp cell culture miniplate (Agilent Technologies, Santa Clara, CA). At ~80–90% confluence, myoblasts were differentiated into myotubes. All experimental procedures were performed at 7 days of differentiation.

Immediately following the procedure, primary muscle cells were isolated
and cultured into myoblast, as previously described (26 (link)). After isolation, myoblasts (Passage 3) were
thawed and grown in a humidified environment with 5% CO₂ at
37°C on a collagen I TC flask (Greiner Bio-one, Monroe, NC). At a
confluence of ~80–90%, myoblasts were subcultured onto either a
6-well type I collagen-coated plate (Corning, Corning, NY) or a 35 mm
collagen-coated glass-bottom dish (MatTek, Ashland, MA) for immunoblot and
fluorescence microscopy, respectively. At ~80–90% confluency,
myoblasts were switched to low-serum (2% horse serum) media to induce
differentiation into myotubes.