Materials and Instruments: All the reagents and solvents are of commercial quality and without further purification. Anhydrous solvent were prepared according to general procedure. Phosphate buffered saline (PBS: 2.97 mM Na2HPO4, 1.05 mM KH2PO4; pH 7.4), 1H and 13C NMR spectra were recorded on a Bruker Advance III 500M spectrometer and referenced to solvent signals. Mass spectra were obtained on Shimadzu LCMS-2020 mass spectrometer. Fluorescence spectra were determined on an Agilent Cary Eclipse spectrophotometer. Absorption spectra were determined on a Hitachi U3010 UV-Visible spectrophotometer. Confocal microscopy images were obtained using confocal microscopy (Leica SP8, Mannheim, Germany). In vivo fluorescence images and photoacoustic images were measured with a Maestro in vivo imaging system (CRI Inc, Woburn, MA) and VisualSonics Vevo® 2100 Imaging System (FUJIFILM VisualSonics Inc.), respectively. Cryostat Section of tissues was performed on Leica CM1900.
Clinical human tissue specimen: The clinical samples of peritoneal, rectal and gastric cancer patients were obtained from Hangzhou First People's Hospital (Hangzhou, China). Written informed consents from patients and approval from the Institutional Research Ethics Committee of the hospital were obtained before the use of these clinical materials for research purposes.
Clinical human tissue specimen: The clinical samples of peritoneal, rectal and gastric cancer patients were obtained from Hangzhou First People's Hospital (Hangzhou, China). Written informed consents from patients and approval from the Institutional Research Ethics Committee of the hospital were obtained before the use of these clinical materials for research purposes.