Lysogeny broth broth

Manufactured by BD

Sourced in United States

Lysogeny Broth (LB) broth is a nutrient-rich medium commonly used in microbiology and molecular biology laboratories for the cultivation of bacteria. It provides the necessary nutrients and growth factors to support the growth and proliferation of a wide range of bacterial species.

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3 protocols using lysogeny broth broth

Synthesis of Mucin-Based Biosensors

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Mucin (from porcine stomach, Type III), 11-mercapto-1-undecanol, epichlorohydrin, diethylene glycol dimethyl ether, potassium chloride, potassium hexacyanoferrate (II) trihydrate, potassium ferricyanide (III), sodium phosphate, and d-mannose were purchased from Sigma-Aldrich (St. Louis, MO, USA). Absolute ethyl alcohol, sodium hydroxide, acetone, sulfuric acid, sodium acetate, acetic acid, and hydrogen peroxide were from DaeJung Chemicals & Metals Co., Ltd. (Gyeonggi-do, Korea). Carboxyfluorescein diacetate (CFDA) was obtained from Dojindo Molecular Technologies, Inc. (Rockville, MD, USA), and potassium phosphate from Pure Chemicals Co., Ltd. (Kyoto, Japan). Lysogeny broth (LB) broth and agar powder were purchased from BD (BD Difco, Lawrence, KS, USA).

Huynh D.T., Kim A.Y, & Kim Y.R. (2017). Identification of Pathogenic Factors in Klebsiella pneumoniae Using Impedimetric Sensor Equipped with Biomimetic Surfaces. Sensors (Basel, Switzerland), 17(6), 1406.

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Bacterial Strain Cultivation and Preparation

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The bacterial strains used in this study are listed in Table 1. Each of these strains was grown in Lysogeny Broth (LB) broth (Difco BD, USA) overnight at 30 °C and 250 rpm. To examine the impact of the treatment by predatory bacteria or violacein, the bacterial culture were prepared by centrifuging with 2,200 × g for 10 min (5430 R, Eppendorf, USA) and resuspended in HEPES buffer (pH 7.2) to an optical density (OD) of 1.0 at 600 nm wavelength. B. bacteriovorus HD100 was cultured as described previously^{33 (link),34}.Table 1

Bacterial strains used in this study.

Bacterial Strains	Characteristics	Ref
S. aureus ATCC 25923
B. cereus ATCC 14579
S. epidermidis ATCC 14990
A. baumannii	Clinical isolate	PNUH^a
E. coli MG1655	Common lab strain; multidrug resistant
K. pneumoniae	Clinical isolate; multidrug resistant	PNUH^a
S. aureus CCARM 3090	Clinical isolate; multidrug resistant	(18)^b

^aClinical strains isolated at the Pusan National University Hospital, Yangsan Campus. ^bObtained from the Culture Collection of Antimicrobial Resistant Microbes (http://knrrb.knrrc.or.kr/index.jsp?rrb=ccarm).

Im H., Choi S.Y., Son S, & Mitchell R.J. (2017). Combined Application of Bacterial Predation and Violacein to Kill Polymicrobial Pathogenic Communities. Scientific Reports, 7, 14415.

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Synthesis of Functionalized Metallic Nanoparticles

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Ready-made phosphate-buffered saline (PBS) solution, Lysogeny Broth (LB) broth (BD Biosciences, San Jose, CA, USA), and 3,3′,5,5′-tetramethylbenzidine (TMB) were purchased from Sigma-Aldrich (St Louis, MO, USA). Cetyltrimethylammonium bromide (CTAB), 3-mercaptopropyl trimethoxysilane (MPTMS), 3-aminopropyldimethyl-ethoxy silane (APDES), ammonium hydroxide (NH₄OH; 28 wt% in water), silver nitrate (AgNO₃), sodium borohydride (NaBH₄), ascorbic acid, ethanol, hydrogen tetrachloroaurate(III) trihydrate (HAuCl₄·3H₂O), methanol, and sulfuric acid (H₂SO₄) were purchased from Sigma-Aldrich.

Ramasamy M., Yi D.K, & An S.S. (2015). Enhanced detection sensitivity of Escherichia coli O157:H7 using surface-modified gold nanorods. International Journal of Nanomedicine, 10(Spec Iss), 179-190.

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