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Mab5005

Manufactured by R&D Systems
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MAB5005 is a monoclonal antibody that binds to and neutralizes human IL-6. It can be used for research purposes.

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Lab products found in correlation

Sc50360, by Santa Cruz Biotechnology (1 mentions) Mab645, by R&D Systems (1 mentions) Tgx stain free fastcast acrylamide kit, by Bio-Rad (1 mentions)

2 protocols using mab5005

1

Immunodetection of Wnt Signaling Proteins

Cited 1 time
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The immunocomplexes bound to the protein G sepharose beads obtained from CM-CITED2 and CM-Ctl as described above, were washed 5 times with PBS1× and immunopurified proteins were analyzed by western blot as previously described27 (link), with a rat monoclonal anti-WNT5A (MAB645, R&D Systems) or rabbit polyclonal anti-WNT11 (SC-50360, Santa Cruz) antibodies used at 1:1000 and 1:200 dilution, respectively. Loading was monitored by staining of PVDF membrane-bound proteins by ponceau s. Western blotting assays performed using 15 μg of protein extracts from zebrafish embryos harvested at 24 and 48 hpf after microinjection of either combined AUG and SPLICING (2.5 ng of each morpholino) or non-injected the control embryos (Control) prepared using conditions previously described27 (link). Proteins were separated by SDS-PAGE on TGX Stain-Free™ FastCast™ Acrylamide Kit (Bio-Rad). CITED2 protein was detected in zebrafish extracts using an anti-CITED2 rat monoclonal IgG2A antibody (MAB5005, R&D System) used at 1:500 dilution as recommended by the manufacturer. Loading control was monitored by detection of total stained proteins transferred to the PVDF membrane.
Santos J.M., Mendes-Silva L., Afonso V., Martins G., Machado R.S., Lopes J.A., Cancela L., Futschik M.E., Sachinidis A., Gavaia P, & Bragança J. (2019). Exogenous WNT5A and WNT11 proteins rescue CITED2 dysfunction in mouse embryonic stem cells and zebrafish morphants. Cell Death & Disease, 10(8), 582.
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2

Quantitative Analysis of CITED2 Expression

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Real-time PCR and Western blotting were performed as previously described (21 (link)). Primer sequences are shown in Supplementary Table 1. CITED2 Western blotting of mouse and human cells and tissue was performed using a polyclonal rat CITED2 antibody (catalog no. MAB5005; R&D Systems). In experiments where samples were prepared in several batches, the control conditions were set at 100% in individual experiments and therefore have no error estimates.
Wang X., Lockhart S.M., Rathjen T., Albadawi H., Sørensen D., O'Neill B.T., Dwivedi N., Preil S.R., Beck H.C., Dunwoodie S.L., Watkins M.T., Rasmussen L.M, & Rask-Madsen C. (2016). Insulin Downregulates the Transcriptional Coregulator CITED2, an Inhibitor of Proangiogenic Function in Endothelial Cells. Diabetes, 65(12), 3680-3690.
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