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Human xl cytokine magnetic luminex performance assay

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The Human XL Cytokine Magnetic Luminex Performance Assay is a multiplex immunoassay that allows for the simultaneous quantitative measurement of multiple human cytokines, chemokines, and growth factors in a single sample. The assay utilizes magnetic beads coated with analyte-specific antibodies and Luminex technology to detect and quantify the target analytes.

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Luminex Performance Assay (13 citations) Magnetic Luminex Assay (62 citations) Luminex assay (130 citations)

4 protocols using human xl cytokine magnetic luminex performance assay

1

Multiplex Cytokine Profiling and Validation

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Stored serum samples were retrieved and thawed at 4°C. For the discovery stage, human 45 cytokines Luminex immunoassays were performed using a 45-plex Luminex assay (Human XL Cytokine Magnetic Luminex Performance Assay, Lot.P276948, R&D Systems, a bio-techne brand) and following the manufacturer’s instructions. The detail containing the 45-plex cytokine assay is presented in Figure 1A. Serum concentrations of each cytokine were measured in pg/ml, and positive control samples for each analyte were assayed in parallel to ensure objective results. For the validation stage, single human cytokine Luminex immunoassays of TRAIL (Human TRAIL/TNFSF10 XL Magnetic Luminex Performance Assay, Lot.LUXLM375, R&D Systems, a bio-techne brand) and IL-10 (Human IL-10 Magnetic Luminex Performance Assay, Lot.LUHM217, R&D Systems, a bio-techne brand) were performed on 288 enrolled patients according to the manufacturer’s instructions. All samples were randomized to locate in plates.
Chen H., Zhou J., Luo J., Wu Y., Qian Y., Shi Y., Qu F., Shi B., Ding J., Cui X, & Yu Y. (2022). Serum multi-cytokines screening identifies TRAIL and IL-10 as probable new biomarkers for prostate health index diagnostic utility adjustment in grey zone aggressive prostate cancer detection: A single-center data in China. Frontiers in Immunology, 13, 901176.
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2

Multiplex Cytokine Profiling Assay

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We used a 45-plex magnetic Ab-coated bead microplate (human XL cytokine magnetic Luminex performance assay, R&D Biosystems, Minneapolis, MN) to measure cytokines, chemokines, and growth factors on the Luminex MAGPIX multiplex platform (Luminex, Austin, TX).
Ostrin E.J., Rider N.L., Alousi A.M., Irajizad E., Li L., Peng Q., Kim S.T., Bashoura L., Arain M.H., Noor L.Z., Patel N., Mehta R., Popat U.R., Hosing C., Jenq R.R., Rondon G., Hanash S.M., Paczesny S., Shpall E.J., Champlin R.E., Dickey B.F, & Sheshadri A. (2023). A Nasal Inflammatory Cytokine Signature Is Associated with Early Graft-versus-Host Disease of the Lung after Allogeneic Hematopoietic Cell Transplantation: Proof of Concept. ImmunoHorizons, 7(6), 421-430.
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3

Multiplex Cytokine Profiling Assay

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We used a 45-plex magnetic Ab-coated bead microplate (human XL cytokine magnetic Luminex performance assay, R&D Biosystems, Minneapolis, MN) to measure cytokines, chemokines, and growth factors on the Luminex MAGPIX multiplex platform (Luminex, Austin, TX).
Lytle D.A., Rice E.W., Johnson C.H, & Fox K.R. (1999). Electrophoretic Mobilities of Escherichia coli O157:H7 and Wild-Type Escherichia coli Strains. Applied and Environmental Microbiology, 65(7), 3222-3225.
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4

BALF Biomarker Quantification Protocol

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BALF was treated with 1% Triton-X100 for 2 hours before samples were used for the specific assays to eliminate all viable virus. D-dimer, soluble tissue factor, tissue type plasminogen activator (tPA), plasminogen activator inhibitor type I (PAI-1), soluble CD40 ligand (sCD40L) and soluble P-selectin (sP- selectin) were measured using Human Thrombosis LEGENDplex™ (#740892, BioLegend, Amsterdam, the Netherlands). Thrombin-antithrombin complexes (TATc) were measured by ELISA (TAT-EIA, Affinity Biologicals, Leiden, the Netherlands). Kallikrein-C1-inhibitor complexes (18 (link)) C3bc (19 (link)) C4bc (20 (link)) mannose binding lectin (MBL) (21 (link)) and C1-inhibitor (C1-INH) antigen (22 (link)) and activity (22 (link)) were measured by assays as described previously. All other mediators and growth factors were measured by Human XL Cytokine Magnetic Luminex Performance Assay (#LKTM014, R&D systems, Abingdon, United Kingdom) and were read on a Bioplex 200.
Nossent E.J., Schuurman A.R., Reijnders T.D., Saris A., Jongerius I., Blok S.G., de Vries H., Duitman J., Vonk Noordegraaf A., Meijboom L.J., Lutter R., Heunks L., Bogaard H.J, & van der Poll T. (2021). Pulmonary Procoagulant and Innate Immune Responses in Critically Ill COVID-19 Patients. Frontiers in Immunology, 12, 664209.
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