Synthetic cannabidiol (CBD; Prati-Donaduzzi, Toledo, PR, Brazil; doses: 3, 10, and 30 mg/10 mL/kg intraperitoneal (i.p.)) stored at 4 °C and protected from light, diluted with sterile saline and 2% polysorbate 80 (Tween® 80; Synth, Diadema, SP, Brazil) for mice [24 (link),25 (link),26 (link)] or synthetic CBD (THC-Pharma, Frankfurt, HE, Germany; doses: 10, 30, and 60 mg/kg/2 mL i.p.) diluted with sterile saline and 3% polysorbate 80 (Tween® 80; Sigma-Aldrich, St. Louise, MO, USA) for systemic administration in FSL rats [24 (link)]. S-ketamine hydrochloride (Pfizer Ltd.a, Ballerup, Denmark; doses: 10, 15, and 20 mg/kg/2 mL i.p.) stored at 4 °C was dissolved in sterile saline [24 (link),105 (link)] and imipramine hydrochloride (IMIP; Abcam, Waltham, MA, USA; dose: 20 mg/kg/10 mL), stored at 4 °C and diluted in sterile saline [106 (link)]. The vehicle (VEH) group received CBD vehicle injections. All drugs were freshly prepared before the experiment. The animals received the treatment randomly by writing down the treatment in pieces of paper, folding them, then drawing one by one for each animal [107 ].
S ketamine hydrochloride
Manufactured by Pfizer
Sourced in Denmark
S-ketamine hydrochloride is a chemical compound used in laboratory settings. It is the hydrochloride salt of the S-enantiomer of ketamine, a dissociative anesthetic. This compound is utilized for research and scientific experimentation purposes, without making claims about its intended use.
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Lab products found in correlation
3 protocols using s ketamine hydrochloride
1
Synthetic CBD and S-Ketamine Dose Evaluation
2
Ketamine Injection in Rat Depression Model
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A single (15 mg/kg) dose of S-Ketamine hydrochloride (5 mg/mL, Pfizer, ATC-code N01AX14) from the local hospital pharmacy was injected i.p. in 12 rats (6 animals in the FSL group and 6 animals in the FRL group) (Muller et al., 2013 (link)), and 12 animals (FSL=6 and FRL=6) received a single i.p. injection of saline. Twenty-four hours after treatment, animals were transcardially perfused as described below.
3
Examining Cardiac Electrical Activity in Anesthetized Rats
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K V 7 channels are expressed in the conduction system and heart muscle fibers (Testai et al., 2016; (link)Hedegaard et al., 2016; (link)Sanguinetti et al., 1996) (link). Therefore, in anesthetized animals, we examined the effect of XE991 compared to a vehicle on the electrical activity of the heart. Rats were anesthetized with S-ketamine hydrochloride (60 mg/ kg; Pfizer, Ballerup Denmark) and xylazine (10 mg/kg; Rompun vet, KVP Pharma + Veterinär produkte Bayer, Kiel Germany) mixture intraperitoneally. ECG was recorded for at least 30 min to ensure normal rhythm; following this, increasing doses of vehicle or XE991 were administered with 30 min intervals in two subgroups of animals. Data were obtained by the use of an animal bio-amplifier (AD Instruments Oxford, United Kingdom), and the software automatically obtained the different ECG values, as previously described (Comerma-Steffensen et al., 2017) (link)., The ECG signals were low-pass filtered by using a standard filter for rats. The rate-corrected QT interval (QTc) was calculated using the standard Bazett's formula (QT C = QT/√RR).
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