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2 protocols using lipoprotein deficient serum lpds

1

Characterization of Lipid-Soluble Compounds

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Trans-resveratrol (98+% pure), 4-Chloro-7-Nitrobenz-2-Oxa-1,3-Diazole (NBD) and 16 DOXYL-stearic acid (16-DSA) were purchased from Sigma Aldrich (St. Louis, MO), potassium iodide (KI) and sodium thiosulfate from Fisher Scientific (Fair Lawn, NJ), and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) from Avanti Polar Lipids (Alabaster, AL). Phospholipid assay kit was from Wako Chemicals USA, Inc. (Richmond, VA), DC and BCA kit for protein assay from BioRad Laboratories (Hercules, CA). Human brain A-172 glioblastoma cells were obtained from ATCC (Manassas, VA), while DMEM, fetal bovine serum (FBS) and lipoprotein deficient serum (LPDS) were from Life Technologies (Grand Island, NY). All solvents used were of analytical grade.
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2

Synthesis and Characterization of Gold Nanoparticles

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Hydrogen tetrachloroaurate (III) (HAuCl4) trihydrate, trisodium citrate, and Tween 20 were purchased from Acros Organics (Morris Plains, NJ, USA); dodecanethiol and tetradecanethiol were obtained from Sigma-Aldrich (St Louis, MO, USA), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) from Avanti Polar Lipids (Alabaster, AL, USA), and the detergent compatible™ (DC™) and the bicinchoninic protein assay kits from Bio-Rad Laboratories (Hercules, CA, USA). Human brain A172 glioblastoma cells were obtained from American Type Culture Collection (Manassas, VA, USA); DMEM, fetal bovine serum (FBS), and lipoprotein-deficient serum (LPDS) were from Life Technologies (Grand Island, NY, USA). Milli-Q water (Millipore, Bedford, MA, USA) was used throughout.
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