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Rna br kit

Manufactured by Qiagen
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The RNA BR kit is a laboratory reagent designed for the purification and extraction of ribonucleic acid (RNA) from biological samples. The kit utilizes a silica-based membrane technology to capture and purify RNA, providing a reliable and efficient method for RNA isolation.

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Lab products found in correlation

Tetro reverse transcription kit, by Meridian Bioscience (3 mentions) Oligo dt 15 mers, by Integrated DNA Technologies (3 mentions) Qubit fluorimeter, by Qiagen (3 mentions) Sybr green mix, by Agilent Technologies (3 mentions) Tissuelyser 2, by Qiagen (3 mentions) Qiazol reagent, by Qiagen (3 mentions) Rnalater, by Thermo Fisher Scientific (3 mentions) Lightcycler 480 2, by Roche (3 mentions)

Spelling variants of this product

Qubit RNA BR Assay Kit (2 citations)

3 protocols using rna br kit

1

Quantitative RT-PCR Analysis of Gene Expression

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Tissue samples stored in RNAlater (Thermo Fisher Scientific) were processed for RNA extraction using a TissueLyser II and QIAzol reagent (QIAGEN). Isolated RNA was quantified using a Qubit fluorimeter and RNA BR kit (QIAGEN). cDNA was synthesized using Tetro reverse transcription kit (Bioline) and oligo dT 15-mers (Integrated DNA Technologies). Quantitative real-time PCR was performed using SYBR™ green mix (Agilent Technologies) and a LightCycler 480 II (Roche). A list of primer sequences used are shown in Table 2. Gene expression levels were determined by second derivative maxima using standard curves (LightCycler software) and expressed relative to the housekeeping gene Rpl13a.
Chenery A.L., Rosini S., Parkinson J.E., Ajendra J., Herrera J.A., Lawless C., Chan B.H., Loke P., MacDonald A.S., Kadler K.E., Sutherland T.E, & Allen J.E. (2021). IL-13 deficiency exacerbates lung damage and impairs epithelial-derived type 2 molecules during nematode infection. Life Science Alliance, 4(8), e202001000.
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2

Comprehensive RNA extraction and quantitative PCR

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Tissue samples stored in RNAlater (Thermo Fisher Scientific) were processed for RNA extraction using a TissueLyser II and QIAzol reagent (Qiagen). Isolated RNA was quantified using a Qubit fluorimeter and RNA BR kit (Qiagen). cDNA was synthesized using Tetro reverse transcription kit (Bioline) and oligo dT 15-mers (Integrated DNA Technologies). Quantitative real-time PCR was performed using SYBR Green mix (Agilent Technologies) and a LightCycler 480 II (Roche). A list of primer sequences used is shown in Table II.
Chenery A.L., Alhallaf R., Agha Z., Ajendra J., Parkinson J.E., Cooper M.M., Chan B.H., Eichenberger R.M., Dent L.A., Robertson A.A., Kupz A., Brough D., Loukas A., Sutherland T.E., Allen J.E, & Giacomin P.R. (2019). Inflammasome-Independent Role for NLRP3 in Controlling Innate Antihelminth Immunity and Tissue Repair in the Lung. The Journal of Immunology Author Choice, 203(10), 2724-2734.
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3

Quantitative RNA Analysis from Tissue Samples

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Tissue samples stored in RNAlater (Thermo Fisher Scientific) were processed for RNA extraction using a TissueLyser II and QIAzol reagent (Qiagen). Isolated RNA was quantified using a Qubit fluorimeter and RNA BR kit (Qiagen). cDNA was synthesized using Tetro reverse transcription kit (Bioline) and oligo dT 15-mers (Integrated DNA Technologies). Quantitative real-time PCR was performed using SYBR green mix (Agilent Technologies) and a LightCycler 480 II (Roche). A list of primer sequences used are shown in Table II.
Chenery A.L., Alhallaf R., Agha Z., Ajendra J., Parkinson J.E., Cooper M.M., Chan B.H., Eichenberger R.M., Dent L.A., Robertson A.A., Kupz A., Brough D., Loukas A., Sutherland T.E., Allen J.E, & Giacomin P.R. (2019). Inflammasome-Independent Role for NLRP3 in Controlling Innate Antihelminth Immunity and Tissue Repair in the Lung. The Journal of Immunology Author Choice, 203(10), 2724-2734.
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