RPMI 1640 medium and fetal bovine serum (FBS) were purchased from Gibco (New York, NY, USA). Hcy, phorbol-12-myristate-13-acetate (PMA) and Oil-red O dye were supplied from Sigma-Aldrich (St. Louis, MO, USA). SBC-115076 and T0901317 was purchased from Selleck Chemicals (Houston, TX, USA). PCSK9 antibody was obtained from Cell Signaling Technology (Danvers, MA, USA). LDLR, ABCG1, CD68, β-actin and horseradish peroxidase (HRP) goat-anti-rabbit antibodies were both obtained from Abcam (Cambridge, MA, USA). ABCA1 antibody was purchased from Novus Biologicals (Littleton, CO, USA). LXRα was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). 22- [- (7- nitrobenz- 2- oxa- 1,3- diazol- 4- yl) amino]- 23, 24- bisnor- 5- cholen- 3β- ol (22-NBD cholesterol) was obtained from Invitrogen (Grand Island, NY, USA). PCR primers were supplied from Sangon Biotech (Shanghai). ApoA-I was supplied from Calbiochem (San Diego, CA, USA) and oxidized-LDL and high-density lipoprotein (HDL) were both obtained from Xiesheng Biotechnology (Beijing).
Pcsk9 antibody
Manufactured by Cell Signaling Technology
Sourced in United States
The PCSK9 antibody is a laboratory reagent designed to detect and quantify the PCSK9 protein. PCSK9 is an enzyme involved in regulating the levels of low-density lipoprotein (LDL) cholesterol in the body. The antibody can be used in various immunoassay techniques to measure PCSK9 levels in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Oil red o dye, by Merck Group (1 mentions)
Sbc 115076, by Selleck Chemicals (1 mentions)
T0901317, by Selleck Chemicals (1 mentions)
Abca1 antibody, by Novus Biologicals (1 mentions)
Abcg1, by Abcam (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Phorbol 12 myristate 13 acetate, by Merck Group (1 mentions)
β actin, by Abcam (1 mentions)
Pcr primers, by Sangon (1 mentions)
C reactive protein (crp), by R&D Systems (1 mentions)
Alexa fluor conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions)
3 protocols using pcsk9 antibody
1
Cholesterol Efflux Assay Protocol
2
Quantification of Signals Using ImageJ
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These techniques were described [37 ]. For quantification of signals ImageJ software was used [41 (link)]. C-reactive protein (CRP) and LDL-receptor antibodies were from R&D Systems. PCSK9 antibody and alpha-smooth muscle actin antibodies (alpha-SMA) were from Cell Signalling Technology (Danvers, MA, USA).
3
Visualizing PCSK9 Expression in oxLDL-Treated Cells
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Following stimulation with various concentrations of oxLDL (50 and 100 µg/ml) for 24 h, the cells were fixed with 4% formaldehyde for 30 min. Following incubation with 0.1% Triton X-100 at room temperature for 30 min, the cells were blocked with goat serum for 1 h at room temperature. After being washed thrice with PBS, the cells were incubated with diluted PCSK9 antibody (1:100; #85813, Cell Signaling Technology, Inc.) at 4°C overnight. After being washed 5 times with PBS, the cells were incubated with Alexa Fluor-conjugated secondary antibody (1:50, A32731, Invitrogen; Thermo Fisher Scientific, Inc.) in the dark for 1 h at room temperature. Finally, the nuclei were stained with 4′,6-diamidino-2-phenylindole (Beyotime Institute of Biotechnology, Inc.) for 5 min at room temperature. After being washed 5 times with PBS, the cells were observed under a fluorescence microscope (IX3; Olympus Corporation).
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