Picoamh elisa kit

AMH assays were performed at the Reproductive Endocrine Research Laboratory at the University of Southern California Keck School of Medicine. AMH was measured primarily using an Ultrasensitive AMH ELISA kit (Ansh Labs, Webster, TX). However, when AMH levels were below the limit of detection of the Ultrasensitive ELISA (<0.07 ng/ml), the picoAMH ELISA kit (Ansh Labs) was used and recovered levels for 83 control samples. The limit of detection of the picoAMH ELISA is 0.003 ng/ml.

All assays were performed at the Reproductive Endocrinology laboratory at the University of Southern California Keck School of Medicine. Case-control trios were analyzed together in the same batch. AMH was measured primarily using an Ultrasensitive AMH ELISA kit (Ansh Labs, Webster, TX). The picoAMH ELISA kit (Ansh Labs) was used when AMH values were below the limit of detection of the Ultrasensitive ELISA (<0.07 ng/ml). The limit of detection of the picoAMH ELISA is 0.003 ng/ml. Of the 1,354 samples, 484 (154 cases, 330 controls) did not have detectable levels by the Ultrasensitive ELISA and were analyzed by the picoAMH ELISA. Of these, 130 (47 cases, 83 controls) had detectable concentrations using the picoAMH. The manufacturer-specified interassay coefficients of variation are as follows: 4.6%, 4.8% and 2.0% at 0.346, 0.715 and 1.85 ng/ml, respectively, for the Ultrasensitive AMH assay, and 4.5%, 2.2% and 3.8% at 22.6, 86.5 and 373 pg/ml, for the picoAMH assays. In our study, blinded control samples (mean=1.89 ng/ml) were run in duplicate in the first 39 batches with interassay CVs of 14.5%. Testosterone was measured by radioimmunoassay with preceding organic solvent extraction and Celite column partition chromatography (16 (link)). The assay sensitivity is 1.5 ng/dL and the interassay CVs are 8%, 12% and 12% at 13, 30 and 96 ng/dL, respectively.