Media, sera and antibiotics for cell culture were from Lonza (Basel, Switzerland). Reagents and substituted piperazines for synthesis of SER, Estradiol and Tamoxifen for cell treatments and all other chemicals were from Sigma-Aldrich (St Louis, MO, USA). TRIzol solution for RNA isolation, SuperScript III Reverse Transcriptase with oligo dT primers for RNA reverse transcription and AmpliTaq Gold for RT-PCR were from Life Technologies (Carlsbad, CA, USA). iTaq Universal SYBR Green Supermix for Quantitative Real-Time PCR (qPCR) was from Biorad (Hercules, CA, USA). 5HT2C and Vinculin antibodies for Western Blot were from Santa Cruz (Dallas, TX, USA). Secondary antibody (Anti-mouse 1:2000) was purchased from Bio-Rad (Hercules, CA, USA).
Trizol solution for rna isolation
Manufactured by Thermo Fisher Scientific
Sourced in United States
TRIzol solution is a ready-to-use reagent designed for the isolation of total RNA from various biological samples. It is a monophasic solution of phenol, guanidine isothiocyanate, and other proprietary components that facilitate the extraction and purification of RNA.
Automatically generated - may contain errors
Lab products found in correlation
Tamoxifen, by Merck Group (2 mentions)
Estradiol, by Merck Group (2 mentions)
Superscript 3 reverse transcriptase with oligo dt primers, by Thermo Fisher Scientific (2 mentions)
Itaq universal sybr green supermix for quantitative real time pcr qpcr, by Bio-Rad (2 mentions)
Amplitaq gold, by Thermo Fisher Scientific (1 mentions)
2 protocols using trizol solution for rna isolation
1
Cell Culture Reagents and Techniques
2
Serotoninergic Receptor Ligand Synthesis and Cell Culture
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Media, sera and antibiotics for cell culture were from Lonza (Basel, Switzerland). Reagents and substituted piperazines for synthesis of serotoninergic receptor ligands, Estradiol and Tamoxifen for cell treatments and all other chemicals were from Sigma-Aldrich (St Louis, MO, USA). TRIzol solution for RNA isolation, SuperScript III Reverse Transcriptase with oligo dT primers for RNA reverse transcription and AmpliTaq Gold for RT-PCR were from Life Technologies (Carlsbad, CA, USA). iTaq Universal SYBR Green Supermix for Quantitative Real-Time PCR (qPCR) was from Biorad (Hercules, CA, USA).
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